School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475000, China.
BMC Genomics. 2024 Sep 19;25(1):883. doi: 10.1186/s12864-024-10790-5.
Tiarella polyphylla D. Don has been traditionally used to cure asthma and skin eruptions. However, the sequence and the structure of the mitogenome of T. polyphylla remained elusive, limiting the genomic and evolution analysis based on the mitogenome.
Using a combination of Illumina and Nanopore sequencing reads, we de novo assembled the complete mitogenome of T. polyphylla. In addition to unveiling the major configuration of the T. polyphylla mitogenome was three circular chromosomes with lengths of 430,435 bp, 126,943 bp, and 55,269 bp, we revealed five (R01-R05) and one (R06) repetitive sequence could mediate the intra- and inter-chromosomal recombination, respectively. Furthermore, we identified 208 short and 25 long tandem segments, seven cp-derived mtDNAs, 106 segments of mtDNAs transferred to the nuclear genome, and 653 predicted RNA editing sites. Based on the sequence of the mitogenomes, we obtained the resolved phylogeny of the seven Saxifragales species.
These results presented the mitogenome features and expanded its potential applications in phylogenetics, species identification, and cytoplasmic male sterility (CMS) in the future.
垂头菊属植物多被传统用于治疗哮喘和皮疹。然而，垂头菊属植物的线粒体基因组的序列和结构仍不清楚，这限制了基于线粒体基因组的基因组和进化分析。
我们使用 Illumina 和 Nanopore 测序reads 的组合，从头组装了垂头菊属植物的完整线粒体基因组。除了揭示垂头菊属植物线粒体基因组的主要构型为三个圆形染色体，长度分别为 430435bp、126943bp 和 55269bp 外，我们还揭示了五个（R01-R05）和一个（R06）重复序列可以分别介导染色体内和染色体间重组。此外，我们鉴定了 208 个短串联重复序列和 25 个长串联重复序列、7 个 cpDNA 衍生的 mtDNAs、106 个 mtDNAs 转移到核基因组的片段，以及 653 个预测的 RNA 编辑位点。基于线粒体基因组序列，我们获得了 7 种虎耳草目物种的解析系统发育关系。
这些结果展示了线粒体基因组的特征，并扩展了其在系统发育学、物种鉴定和细胞质雄性不育（CMS）方面的潜在应用。
