Matranga Valeria, Di Ferrol Daniela, Zito Francesca, Cervello Melchiorre, Nakano Eizo
Istituto di Biologia dello Sviluppo del CNR, Via Archirafi 20, I-90123, Palermo, Italy.
Department of Biology, Nagoya University, 464, Chikusa, Nagoya, Japan.
Rouxs Arch Dev Biol. 1992 May;201(3):173-178. doi: 10.1007/BF00188716.
A new embryonic extracellular matrix protein has been purified from eggs of the sea urchin Paracentrotus lividus. The molecule is a 210 kD dimer consisting of two 105 kD subunits that are held together by S-S bridges. In the unfertilized egg, the protein is found within granules uniformly distributed throughout the cytoplasm. After the egg is fertilized, the antigen is polarized to the apical surface of ectodermal and endodermal cells during all of the developmental stages examined, until the pluteus larva is formed. The protein promotes the adhesion of blastula cells to the substrate and is antigenically distinct from echinonectin, a well characterized substrate adhesion molecule. This report adds a new candidate to the list of known extracellular matrix molecules for the regulation of differentiation and morphogenesis in the sea urchin embryo.
一种新的胚胎细胞外基质蛋白已从紫球海胆的卵中纯化出来。该分子是一个210 kD的二聚体，由两个105 kD的亚基组成，这两个亚基通过S-S桥连接在一起。在未受精的卵中，该蛋白存在于均匀分布于整个细胞质中的颗粒内。卵受精后，在所有检查的发育阶段，该抗原都会极化到外胚层和内胚层细胞的顶端表面，直到形成长腕幼虫。该蛋白促进囊胚细胞与底物的黏附，并且在抗原性上与棘皮粘连蛋白不同，棘皮粘连蛋白是一种特征明确的底物黏附分子。本报告为已知的细胞外基质分子列表增添了一个新的候选分子，用于调控海胆胚胎的分化和形态发生。
