Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG.
EMBO J. 1987 Aug;6(8):2197-202. doi: 10.1002/j.1460-2075.1987.tb02490.x.
The complete nucleotide sequence of the gene encoding the cytochrome subunit of the photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis, and the derived amino acid sequence are presented. The nucleotide sequence of the gene reveals the existence of a typical bacterial signal peptide of 20 amino acid residues which is not found in the mature cytochrome subunit. The gene encoding the cytochrome subunit is preceded by the gene encoding the M subunit. Both genes overlap by 1 bp. The mature cytochrome subunit consists of 336 amino acid residues; 73% of its amino acid sequence was confirmed by protein sequencing work. The mol. wt of the cytochrome subunit including the covalently bound fatty acids and the bound heme groups is 40 500. The internal sequence homology is low, despite the symmetric structure of the cytochrome subunit previously shown by X-ray crystallographic analysis of the intact photosynthetic reaction centre. Sequence homologies to other cytochromes were not found.
给出了编码光合反应中心细胞色素亚基的基因的完整核苷酸序列和推导的氨基酸序列。该基因的核苷酸序列揭示了存在典型的 20 个氨基酸残基的细菌信号肽，而成熟的细胞色素亚基中不存在该信号肽。编码细胞色素亚基的基因位于编码 M 亚基的基因之前。两个基因重叠 1bp。成熟的细胞色素亚基由 336 个氨基酸残基组成；其氨基酸序列的 73%已通过蛋白质测序工作得到证实。包括共价结合的脂肪酸和结合的血红素基团在内的细胞色素亚基的分子量为 40500。尽管以前通过完整光合反应中心的 X 射线晶体学分析表明细胞色素亚基具有对称结构，但内部序列同源性较低。未发现与其他细胞色素的序列同源性。
