Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium.
EMBO J. 1987 Sep;6(9):2513-8. doi: 10.1002/j.1460-2075.1987.tb02537.x.
Phosphinothricin (PPT) is a potent inhibitor of glutamine synthetase in plants and is used as a non-selective herbicide. The bar gene which confers resistance in Streptomyces hygroscopicus to bialaphos, a tripeptide containing PPT, encodes a phosphinothricin acetyltransferase (PAT) (see accompanying paper). The bar gene was placed under control of the 35S promoter of the cauliflower mosaic virus and transferred to plant cells using Agrobacterium-mediated transformation. PAT was used as a selectable marker in protoplast co-cultivation. The chimeric bar gene was expressed in tobacco, potato and tomato plants. Transgenic plants showed complete resistance towards high doses of the commercial formulations of phosphinothricin and bialaphos. These data present a successful approach to obtain herbicide-resistant plants by detoxification of the herbicide.
膦丝菌素（PPT）是一种植物中谷氨酰胺合成酶的有效抑制剂，被用作非选择性除草剂。潮霉素抗性基因来源于吸水链霉菌，能够赋予其对包含 PPT 的双丙氨膦的抗性，该基因编码膦丝菌素乙酰转移酶（PAT）（见相关论文）。bar 基因受到花椰菜花叶病毒 35S 启动子的调控，并通过根癌农杆菌介导的转化转移到植物细胞中。PAT 被用作原生质体共培养中的选择标记。嵌合 bar 基因在烟草、马铃薯和番茄植物中表达。转基因植物对高剂量的膦丝菌素和双丙氨膦的商业制剂表现出完全抗性。这些数据提供了一种通过解毒除草剂获得抗除草剂植物的成功方法。
