Biogen S. A., 46 Route des Acacias, CH-1227 Geneva, Switzerland.
EMBO J. 1987 Sep;6(9):2519-23. doi: 10.1002/j.1460-2075.1987.tb02538.x.
A gene which confers resistance to the herbicide bialaphos (bar) has been characterized. The bar gene was originally cloned from Streptomyces hygroscopicus, an organism which produces the tripeptide bialaphos as a secondary metabolite. Bialaphos contains phosphinothricin, an analogue of glutamate which is an inhibitor of glutamine synthetase. The bar gene product was purified and shown to be a modifying enzyme which acetylates phosphinothricin or demethylphosphinothricin but not bialaphos or glutamate. The bar gene was subcloned and its nucleotide sequence was determined. Interspecific transfer of this Streptomyces gene into Escherichia coli showed that it could be used as a selectable marker in other bacteria. In the accompanying paper, bar has been used to engineer herbicide-resistant plants.
已鉴定出一种可赋予草丁膦（bar）除草剂抗性的基因。bar 基因最初是从吸水链霉菌中克隆出来的，吸水链霉菌是一种产生作为次级代谢产物的三肽草丁膦的生物体。草丁膦含有草丁膦，一种谷氨酸类似物，是谷氨酰胺合成酶的抑制剂。bar 基因产物被纯化并被证明是一种修饰酶，它乙酰化草丁膦或去甲基草丁膦，但不作用于草丁膦或谷氨酸。bar 基因被亚克隆并确定了其核苷酸序列。该链霉菌基因在种间转移到大肠杆菌中表明，它可在其他细菌中用作可选择的标记。在随附的论文中，bar 已被用于工程化抗除草剂植物。
