Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
EMBO J. 1989 Aug;8(8):2251-5. doi: 10.1002/j.1460-2075.1989.tb08349.x.
The 33 kd protein of the photosynthetic oxygen-evolving complex is synthesized in the cytoplasm as a larger precursor and transported into the thylakoid lumen via a stromal intermediate form. In this report we describe a reconstituted system in which the later stages of this import pathway can be studied in isolation. We demonstrate import of the 33 kd protein, probably as the intermediate form, into isolated pea thylakoids by a mechanism which is stimulated by the addition of ATP. The imported protein is processed to the mature size and is resistant to digestion by proteases. The thylakoidal protein transport system is specific in that non-chloroplast proteins and precursors of stromal proteins are not imported.
光合放氧复合体的 33kDa 蛋白在细胞质中作为较大的前体合成，并通过基质中间形式运入类囊体腔。在本报告中，我们描述了一个再组成的系统，在此系统中可以分离研究此输入途径的后期阶段。我们证明 33kDa 蛋白可能作为中间形式通过依赖于 ATP 加入的机制输入到分离的豌豆类囊体中。输入的蛋白被加工成熟型并且对蛋白酶消化有抗性。类囊体蛋白运输系统是特异的，因为非叶绿体蛋白和基质蛋白前体不能被输入。
