Eneroth A, Kristensson K, Ljungdahl A, Olsson T
Department of Cellular and Neuropathology, Huddinge Hospital, Stockholm, Sweden.
J Neurocytol. 1991 Mar;20(3):225-31. doi: 10.1007/BF01186995.
Interferon-gamma-like immunoreactivity was observed in a subpopulation of 16-day-old embryonic rat spinal ganglion neurons using two monoclonal antibodies directed against different epitopes of recombinant interferon-gamma. During ontogenesis both in vivo and in vitro, it was found that the strong immunoreactivity was confined to small neurons when neurons become morphologically distinct on the basis of size. In vivo, the interferon-gamma-immunoreactive neurons started to express major histocompatibility complex class I antigens after the first postnatal week, while in vitro no such antigen could be detected. A quantitative Elisa method was developed to determine the levels of major histocompatibility complex class I and interferon-gamma in vitro, whereby increased amounts of major histocompatibility complex class I antigen was detected after exposing the cultures to recombinant interferon-gamma and Sendai virus. Sendai virus also caused a small increase in interferon-gamma with a peak about 12 hours after infection. The in vitro system will be used to study further the role of the putative neuronal interferon-gamma-like molecule in the regulation of cell growth, for induction of major histocompatibility complex antigens and in virus infection of sensory neurons.
使用两种针对重组干扰素-γ不同表位的单克隆抗体，在16日龄胚胎大鼠脊髓神经节神经元的一个亚群中观察到了干扰素-γ样免疫反应性。在体内和体外的个体发育过程中，发现当神经元在大小基础上形态变得明显时，强免疫反应性局限于小神经元。在体内，产后第一周后，干扰素-γ免疫反应性神经元开始表达主要组织相容性复合体I类抗原，而在体外未检测到此类抗原。开发了一种定量酶联免疫吸附测定（ELISA）方法来测定体外主要组织相容性复合体I类和干扰素-γ的水平，由此在将培养物暴露于重组干扰素-γ和仙台病毒后，检测到主要组织相容性复合体I类抗原量增加。仙台病毒还导致干扰素-γ略有增加，感染后约12小时达到峰值。体外系统将用于进一步研究假定的神经元干扰素-γ样分子在细胞生长调节、主要组织相容性复合体抗原诱导以及感觉神经元病毒感染中的作用。
