Olsson T, Kristensson K, Ljungdahl A, Maehlen J, Holmdahl R, Klareskog L
Department of Neurology, Karolinska Institutet, Huddinge, Sweden.
J Neurosci. 1989 Nov;9(11):3870-5. doi: 10.1523/JNEUROSCI.09-11-03870.1989.
Major histocompatibility complex (MHC) encoded antigens, absent or present at only low levels in normal brain tissue, are induced locally around axotomized motor neurons. We here report that immunoreactivity for the potent MHC-inducing factor, gamma-interferon (IFN-gamma), appears in the cytoplasm of such neurons. Rat facial nerves were interrupted (crushed or cut), and later, at various intervals, cryosections from the facial nuclei were subjected to immunohistochemistry with monoclonal antibodies reacting with IFN-gamma and MHC antigens. IFN-gamma-like immunoreactivity appeared briskly in the cytoplasm of the axotomized motor neurons. The immunoreactivity subsided after a nerve crush as the target muscles were reinnervated, but persisted when nerve regeneration was prevented after a nerve cut. These results point to a role for nerve cell-derived IFN-gamma-like molecules in eliciting the neural cell responses to axotomy.
主要组织相容性复合体（MHC）编码的抗原在正常脑组织中不存在或仅以低水平存在，却在轴突切断的运动神经元周围局部诱导产生。我们在此报告，强效MHC诱导因子γ干扰素（IFN-γ）的免疫反应性出现在此类神经元的细胞质中。将大鼠面神经切断（挤压或切断），随后在不同时间间隔，对面神经核的冰冻切片用与IFN-γ和MHC抗原反应的单克隆抗体进行免疫组织化学检测。轴突切断的运动神经元细胞质中迅速出现IFN-γ样免疫反应性。神经挤压后，随着靶肌肉重新获得神经支配，免疫反应性消退，但在神经切断后阻止神经再生时，免疫反应性持续存在。这些结果表明神经细胞衍生的IFN-γ样分子在引发神经细胞对轴突切断的反应中发挥作用。
