Kemppainen Robert J, Birchfield Jeremy R
Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
Am J Vet Res. 2006 Feb;67(2):259-65. doi: 10.2460/ajvr.67.2.259.
To compare results obtained from assay of total thyroxine (T4) concentration in serum of dogs and cats by use of 4 methods.
Serum samples obtained from 98 dogs and 100 cats and submitted by veterinarians to an endocrine testing laboratory.
Total T4 concentration was determined in each sample by use of 4 assay methods. Assay methods included a radioimmunoassay (RIA) marketed for use in dogs, an RIA for use in humans, a chemiluminescent enzyme immunoassay for use in humans, and an in-house ELISA.
Total T4 concentrations obtained by use of all methods were significantly correlated. Bias-plot comparison revealed similar good overall agreement. Total T4 concentrations determined by use of the RIA marketed for use in dogs were generally lower than concentrations measured by use of the other methods. Clinical comparisons were made by evaluation of the T4 results in the context of the reference range recommended by each laboratory. A difference was found for clinical comparisons on the basis of T4 assay method when used to identify dogs as possible hypothyroid suspects. This difference was related more to the reference range used than to the absolute T4 value. The number of hyperthyroid-suspect cats with T4 values greater than the reference range was the same for each of the 4 assay methods.
Total T4 concentrations determined in dogs and cats by use of 4 commonly used methods provided similar and consistent results.
比较使用4种方法测定犬猫血清中总甲状腺素（T4）浓度所获得的结果。
从98只犬和100只猫采集的血清样本，由兽医提交至一家内分泌检测实验室。
使用4种检测方法测定每个样本中的总T4浓度。检测方法包括一种市售的用于犬的放射免疫分析（RIA）、一种用于人的RIA、一种用于人的化学发光酶免疫分析以及一种内部酶联免疫吸附测定（ELISA）。
使用所有方法获得的总T4浓度显著相关。偏差图比较显示总体一致性良好。使用市售的用于犬的RIA测定的总T4浓度通常低于使用其他方法测得的浓度。通过在每个实验室推荐的参考范围内评估T4结果进行临床比较。当用于识别可能患有甲状腺功能减退的犬时，基于T4检测方法的临床比较存在差异。这种差异更多地与所使用的参考范围有关，而非与绝对T4值有关。对于4种检测方法中的每一种，T4值高于参考范围的疑似甲状腺功能亢进的猫的数量相同。
使用4种常用方法测定犬猫的总T4浓度可提供相似且一致的结果。
