Mei Z T, Duan S H
Shanghai Institute of Physiology, Academia Sinica.
Sheng Li Xue Bao. 1991 Feb;43(1):8-13.
Using the method of bilateral injection of GABA (100 micrograms/microliters) and muscimol (0.1 micrograms/microliters) into the caudate-putamen nucleus (CPN) we have studied the role of GABA-ergic transmission of CPN in the control of conditioned behavior. In order to compare the effects of GABA and muscimol on two behavioral paradigms, rats of either sex weighing 160-180 g were trained to perform discrimination learning and conditioned avoidance response. A tone (1 kHz, 80 dB) was used as a conditioned stimulus. After bilateral injections of GABA and muscimol into the trained rats' CPN respectively, the conditioned avoidance response was inhibited temporarily, whereas the discrimination learning remained almost unchanged. In addition, the inhibitory effect of muscimol on the conditioned avoidance response was attenuated or even abolished by following bilateral intra-CPN injection of picrotoxin (0.1 micrograms/microliters for each side). As a control, the same volume of saline was injected into CPN bilaterally, and it showed that there was no change of both the conditioned avoidance response and the discrimination learning. The results show that the GABA-ergic transmission of CPN does play a role in the control of avoidance conditioning in rats, and thus support the suggestion that GABA-ergic transmission of the caudate-globus pallidus plays an important role in the control of conditioned behavior.
采用向尾状核-壳核（CPN）双侧注射γ-氨基丁酸（GABA，100微克/微升）和蝇蕈醇（0.1微克/微升）的方法，我们研究了CPN的γ-氨基丁酸能传递在条件行为控制中的作用。为了比较GABA和蝇蕈醇对两种行为范式的影响，对体重160 - 180克的雌雄大鼠进行训练，使其进行辨别学习和条件性回避反应。使用音调（1千赫，80分贝）作为条件刺激。分别向训练后的大鼠CPN双侧注射GABA和蝇蕈醇后，条件性回避反应被暂时抑制，而辨别学习几乎没有变化。此外，在CPN双侧注射印防己毒素（每侧0.1微克/微升）后，蝇蕈醇对条件性回避反应的抑制作用减弱甚至消失。作为对照，向CPN双侧注射相同体积的生理盐水，结果显示条件性回避反应和辨别学习均无变化。结果表明，CPN的γ-氨基丁酸能传递在大鼠回避条件反射的控制中确实发挥作用，从而支持了尾状核-苍白球的γ-氨基丁酸能传递在条件行为控制中起重要作用的观点。
