分化中的U937细胞中肌动蛋白聚合的调节与百日咳毒素敏感的G蛋白Gi2的膜水平升高相关。
The regulation of actin polymerization in differentiating U937 cells correlates with increased membrane levels of the pertussis-toxin-sensitive G-protein Gi2.
作者信息
Sheth B, Banks P, Burton D R, Monk P N
机构信息
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, U.K.
出版信息
Biochem J. 1991 May 1;275 ( Pt 3)(Pt 3):809-11. doi: 10.1042/bj2750809.
Abstract
Undifferentiated U937 cells appear to lack a capacity of increase cellular F-actin. However, electropermeabilized cells gain the ability to respond in this way to a guanine nucleotide analogue, guanosine 5'-[gamma-thio]trisphosphate (GTP[S]) after 1 h of treatment with dibutyryl cyclic AMP (db-cAMP). The results reported here show that the levels of membrane association of the G-protein Gi2 alpha increase with a time course identical with that of the GTP[S]-sensitivity of electropermeabilized cells. These results suggest that Gi2 alpha may be involved in the signal-transduction pathway leading to actin polymerization in db-cAMP-differentiated U937 cells.
摘要
未分化的U937细胞似乎缺乏增加细胞F-肌动蛋白的能力。然而，用电穿孔法处理的细胞在用二丁酰环磷腺苷（db-cAMP）处理1小时后，获得了以这种方式对鸟嘌呤核苷酸类似物鸟苷5'-[γ-硫代]三磷酸（GTP[S]）作出反应的能力。此处报道的结果表明，G蛋白Gi2α的膜结合水平随时间的变化过程与电穿孔细胞对GTP[S]的敏感性相同。这些结果表明，Gi2α可能参与了导致db-cAMP分化的U937细胞中肌动蛋白聚合的信号转导途径。
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本文引用的文献
1
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J Cell Biol. 1985 Sep;101(3):1161-6. doi: 10.1083/jcb.101.3.1161.
2
Recruitment of actin to the cytoskeletons of human monocyte-like cells activated by complement fragment C5a. Is protein kinase C involved?补体片段C5a激活的人单核细胞样细胞细胞骨架中肌动蛋白的募集。蛋白激酶C是否参与其中？
Biochem J. 1988 Jun 15;252(3):765-9. doi: 10.1042/bj2520765.
3
Roles of G protein subunits in transmembrane signalling.G蛋白亚基在跨膜信号传导中的作用。
Nature. 1988 May 12;333(6169):129-34. doi: 10.1038/333129a0.
4
Myristoylated alpha subunits of guanine nucleotide-binding regulatory proteins.
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7493-7. doi: 10.1073/pnas.84.21.7493.
5
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Biochem Biophys Res Commun. 1985 Feb 15;126(3):1174-81. doi: 10.1016/0006-291x(85)90309-2.
6
Evidence that de novo protein synthesis participates in a time-dependent augmentation of the chemotactic peptide-induced respiratory burst in neutrophils. Effects of recombinant human colony stimulating factors and dihydrocytochalasin B.
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7
8
9
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