Boyne Michael T, Pesavento James J, Mizzen Craig A, Kelleher Neil L
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
J Proteome Res. 2006 Feb;5(2):248-53. doi: 10.1021/pr050269n.
Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were alpha-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding approximately 10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.
自上而下的分析表明，至少有14个编码组蛋白H2A的基因在HeLa细胞中共表达。对这些种类的表征显示，除了H2A.Z和H2A.F/Z之外，所有的都进行了α-N-乙酰化，H2A.O和H2A.C、D、I、N、P最为丰富，而那些丰度超过约10%的则缺乏翻译后修饰。对H2A形式的这种明确鉴定说明了自上而下质谱分析的优势，并提供了通过细胞周期对H2A调控的全局视角。
