Paulraj R, Behari J
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Mutat Res. 2006 Apr 11;596(1-2):76-80. doi: 10.1016/j.mrfmmm.2005.12.006. Epub 2006 Feb 2.
This investigation concerns with the effect of low intensity microwave (2.45 and 16.5 GHz, SAR 1.0 and 2.01 W/kg, respectively) radiation on developing rat brain. Wistar rats (35 days old, male, six rats in each group) were selected for this study. These animals were exposed for 35 days at the above mentioned frequencies separately in two different exposure systems. After the exposure period, the rats were sacrificed and the whole brain tissue was dissected and used for study of single strand DNA breaks by micro gel electrophoresis (comet assay). Single strand DNA breaks were measured as tail length of comet. Fifty cells from each slide and two slides per animal were observed. One-way ANOVA method was adopted for statistical analysis. This study shows that the chronic exposure to these radiations cause statistically significant (p<0.001) increase in DNA single strand breaks in brain cells of rat.
本研究关注低强度微波（分别为2.45和16.5吉赫兹，比吸收率分别为1.0和2.01瓦/千克）辐射对发育中大鼠大脑的影响。本研究选用了Wistar大鼠（35日龄，雄性，每组6只大鼠）。这些动物在两个不同的暴露系统中分别于上述频率下暴露35天。暴露期结束后，处死大鼠，解剖整个脑组织，用于通过微凝胶电泳（彗星试验）研究单链DNA断裂情况。单链DNA断裂以彗星的尾长来衡量。观察每只动物的两张载玻片，每张载玻片观察50个细胞。采用单因素方差分析方法进行统计分析。本研究表明，长期暴露于这些辐射会导致大鼠脑细胞中的DNA单链断裂出现具有统计学意义的增加（p<0.001）。
