Nienhuijs Marloes E L, Walboomers X Frank, Merkx Matthias A W, Stoelinga Paul J W, Jansen John A
Department of Oral and Maxillofacial Surgery, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
Biomaterials. 2006 Jun;27(16):3109-14. doi: 10.1016/j.biomaterials.2006.01.021. Epub 2006 Feb 3.
COLLOSS, a bovine extracellular matrix product containing native BMPs has already shown osteoinductive properties. To overcome problems with risk of transmissable spongiform encephalopathy (TSE) infection, an equine derived version was investigated in this study, named COLLOSS E. Disc- and tube-shaped implants were made from titanium fibre mesh. The central space of tubes was filled and the discs were impregnated with the COLLOSS E material to assess osteo-induction. These implants and non-loaded controls were implanted subcutaneously into the back of Wistar rats. After implantation periods of 2, 8, and 12 weeks, the implants were retrieved and sections were made. Histology showed a thin fibrous capsule surrounding the titanium mesh and a very mild tissue reaction. The disc implants, loaded or non-loaded, showed no bone formation at all. After 2 weeks of implantation 3 out of 5 of the loaded tubes showed bone formation with a mean of 0.3 mm2 areas of new formed bone, after 8 weeks 3 out of 6 and 0.7 mm2, and after 12 weeks this increased to 6 out of 6 and 1.0 mm2. In the non-loaded tubes only connective tissue in growth was seen. In conclusion, it was demonstrated that COLLOSS E material, loaded in a titanium fibre mesh tube shows osteoinductive properties. The effect of COLLOSS E has to be investigated further in orthotopic sites, which resemble more the final clinical application for bone reconstruction.
COLLOSS是一种含有天然骨形态发生蛋白（BMPs）的牛细胞外基质产品，已显示出骨诱导特性。为克服可传播性海绵状脑病（TSE）感染风险问题，本研究对一种马源版本进行了研究，命名为COLLOSS E。盘状和管状植入物由钛纤维网制成。将管状植入物的中心空间填充，盘状植入物用COLLOSS E材料浸渍，以评估骨诱导作用。将这些植入物和未加载的对照皮下植入Wistar大鼠背部。在植入2、8和12周后，取出植入物并制作切片。组织学显示钛网周围有一层薄的纤维囊和非常轻微的组织反应。加载或未加载的盘状植入物均未显示出任何骨形成。植入2周后，5个加载的管状植入物中有3个显示出骨形成，新形成骨的平均面积为0.3平方毫米；8周后，6个中有3个，面积为0.7平方毫米；12周后，增加到6个中有6个，面积为1.0平方毫米。在未加载的管状植入物中，仅见结缔组织生长。总之，结果表明，加载在钛纤维网管中的COLLOSS E材料显示出骨诱导特性。COLLOSS E的效果必须在原位进一步研究，原位更类似于骨重建的最终临床应用。
