Erasto P, Grierson D S, Afolayan A J
Department of Botany, University of Fort Hare, Alice 5700, South Africa.
J Ethnopharmacol. 2006 Jun 15;106(1):117-20. doi: 10.1016/j.jep.2005.12.016. Epub 2006 Feb 3.
Phytochemical analysis of the leaves of Vernonia amygdalina yielded two known sesquiterpene lactones: vernolide and vernodalol. The two compounds were tested by agar dilution method against 10 bacteria strains and 5 fungi species. Both compounds exhibited a significant bactericidal activity against five Gram positive bacteria while lacking efficacy against the Gram negative strains. In the antifungal test, while vernolides exhibited high activity with LC(50) values of 0.2, 0.3 and 0.4 mg/ml against Penicillium notatum, Aspergillus flavus, Aspergillus niger and Mucor hiemalis, respectively, vernodalol showed moderate inhibitions against Aspergillus flavus, Penicillium notatum and Aspergillus niger with LC(50) values of 0.3, 0.4 and 0.5 mg/ml, respectively. Both compounds were ineffective against Fusarium oxysporum, a microbe known to be highly resistant to chemical agents. However, the antimicrobial results of this study correspond positively with the claimed ethnomedical uses of the leaves of Vernonia amygdalina in the treatment of various infectious diseases.
斑鸠菊内酯和斑鸠菊醇。采用琼脂稀释法对这两种化合物针对10种细菌菌株和5种真菌进行了测试。两种化合物对5种革兰氏阳性菌均表现出显著的杀菌活性，而对革兰氏阴性菌菌株则无效。在抗真菌试验中，斑鸠菊内酯对分别对青霉、黄曲霉、黑曲霉和冬毛霉表现出高活性，LC(50)值分别为0.2、0.3和0.4mg/ml，而斑鸠菊醇对黄曲霉、青霉和黑曲霉表现出中等抑制作用，LC(50)值分别为0.3、0.4和0.5mg/ml。两种化合物对尖孢镰刀菌均无效，尖孢镰刀菌是一种已知对化学药剂高度耐药的微生物。然而，本研究的抗菌结果与扁桃斑鸠菊叶子在治疗各种传染病方面的民族医学应用声称呈正相关。
