Chan Wai-Yee, Wu Shao-Ming, Ruszczyk Lisa, Law Evelyn, Lee Tin-Lap, Baxendale Vanessa, Lap-Yin Pang Alan, Rennert Owen M
Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Building 49, Room 2A08, 49 Convent Drive, MSC 4429, Bethesda, MD 20892-4429, USA.
Genomics. 2006 Jun;87(6):681-92. doi: 10.1016/j.ygeno.2005.12.006. Epub 2006 Feb 3.
Computational analyses have identified the widespread occurrence of antisense transcripts in the human and the mouse genome. However, the structure and the origin of the majority of the antisense transcripts are unknown. The presence of antisense transcripts for 19 of 64 differentially expressed genes during mouse spermatogenesis was demonstrated with orientation-specific RT-PCR. These antisense transcripts were derived from a wide variety of origins, including processed sense transcripts, intronic and exonic sequences of a single gene or multiple genes, intergenic sequences, and pseudogenes. They underwent normal and alternative splicing, 5' capping, and 3' polyadenylation, similar to the sense transcripts. There were also antisense transcripts that were not capped and/or polyadenylated. The testicular levels of the sense transcripts were higher than those of the antisense transcripts in all cases, while the relative expression in nontesticular tissues was variable. Thus antisense transcripts have complex origins and structures and the sense and antisense transcripts can be regulated independently.
计算分析已确定在人类和小鼠基因组中广泛存在反义转录本。然而，大多数反义转录本的结构和起源尚不清楚。通过定向特异性逆转录聚合酶链反应（RT-PCR）证实了在小鼠精子发生过程中64个差异表达基因中有19个存在反义转录本。这些反义转录本来源于多种起源，包括加工后的正义转录本、单个基因或多个基因的内含子和外显子序列、基因间序列和假基因。它们经历了正常和可变剪接、5'加帽和3'多聚腺苷酸化，类似于正义转录本。也有未加帽和/或未进行多聚腺苷酸化的反义转录本。在所有情况下，正义转录本的睾丸水平均高于反义转录本，而非睾丸组织中的相对表达则有所不同。因此，反义转录本具有复杂的起源和结构，正义和反义转录本可以独立调控。
