Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Bioinformatics. 2010 Feb 15;26(4):585-6. doi: 10.1093/bioinformatics/btp695. Epub 2009 Dec 21.
Serial analysis of gene expression (SAGE) provides an alternative, with additional advantages, to microarray gene expression studies. GonadSAGE is the first publicly available web-based SAGE database on male gonad development that covers six male mouse embryonic gonad stages, including E10.5, E11.5, E12.5, E13.5, E15.5 and E17.5. The sequence coverage of each SAGE library is beyond 150K, 'which is the most extensive sequence-based male gonadal transcriptome to date'. An interactive web interface with customizable parameters is provided for analyzing male gonad transcriptome information. Furthermore, the data can be visualized and analyzed with the other genomic features in the UCSC genome browser. It represents an integrated platform that leads to a better understanding of male gonad development, and allows discovery of related novel targets and regulatory pathways.
基因表达系列分析（SAGE）为基因表达研究提供了一种替代方法，具有额外的优势。性腺 SAGE 是第一个公开的基于网络的雄性性腺发育 SAGE 数据库，涵盖了六个雄性小鼠胚胎性腺阶段，包括 E10.5、E11.5、E12.5、E13.5、E15.5 和 E17.5。每个 SAGE 文库的序列覆盖率都超过 150K，“这是迄今为止最广泛的基于序列的雄性性腺转录组”。提供了一个带有可定制参数的交互式网络界面，用于分析雄性性腺转录组信息。此外，还可以使用 UCSC 基因组浏览器中的其他基因组特征来可视化和分析数据。它代表了一个集成平台，有助于更好地理解雄性性腺发育，并允许发现相关的新靶点和调控途径。
