Brown K H, Lee R W, Thorgaard G H
School of Biological Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA, USA.
Comp Biochem Physiol B Biochem Mol Biol. 2006 Apr;143(4):415-21. doi: 10.1016/j.cbpb.2005.12.015. Epub 2006 Feb 3.
Chromosome set manipulation was used to produce rainbow trout, Oncorhynchus mykiss, with identical nuclear backgrounds, but different maternal backgrounds to determine mitochondrial effects on development rate and oxygen consumption. Significant differences in development rate and oxygen consumption were observed between groups from different females. Development rates ranged from a mean of 317.97 degree days ( degrees d) to 335.25 degrees d in progeny from different females. Mean oxygen consumption rates ranged from 3.31 micromol O2 g(-1) wet mass h(-1) to 9.66 micromol O2 g(-1) wet mass h(-1). Oxygen consumption and development rate analysis revealed the two slowest developing groups had the highest oxygen consumption rates. Development rate differences between second generation clonal females indicate that mitochondrial genomes play a significant role on early development and are comparable to development rate differences between clonal lines of rainbow trout. These results indicate that selection for mitochondrial genomes could increase growth rates and possibly food conversion ratios in aquaculture species.
通过染色体组操作培育出了具有相同核背景但母本背景不同的虹鳟（Oncorhynchus mykiss），以确定线粒体对发育速率和耗氧量的影响。在来自不同雌鱼的组间观察到发育速率和耗氧量存在显著差异。不同雌鱼后代的发育速率范围为平均317.97度日（℃·d）至335.25℃·d。平均耗氧率范围为3.31微摩尔O₂克⁻¹湿重小时⁻¹至9.66微摩尔O₂克⁻¹湿重小时⁻¹。耗氧量和发育速率分析表明，发育最慢的两个组具有最高的耗氧率。第二代克隆雌鱼之间的发育速率差异表明，线粒体基因组在早期发育中起重要作用，并且与虹鳟克隆系之间的发育速率差异相当。这些结果表明，选择线粒体基因组可以提高水产养殖物种的生长速率，并可能提高食物转化率。
