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利用紫外线照射对欧洲鲈鱼（Dicentrarchus labrax L.）鱼卵进行基因灭活：观察与展望

Genetic inactivation of European sea bass (Dicentrarchus labrax L.) eggs using UV-irradiation: observations and perspectives.

作者信息

Colléter Julie, Penman David J, Lallement Stéphane, Fauvel Christian, Hanebrekke Tanja, Osvik Renate D, Eilertsen Hans C, D'Cotta Helena, Chatain Béatrice, Peruzzi Stefano

机构信息

Cirad, Persyst, UMR Intrepid, Campus International de Baillarguet, Montpellier, France; Ifremer, Laboratoire de Recherche Piscicole en Méditerranée, Station Expérimentale d'Aquaculture, Palavas Les Flots, France.

University of Stirling, Institute of Aquaculture, Stirling, Scotland.

出版信息

PLoS One. 2014 Oct 20;9(10):e109572. doi: 10.1371/journal.pone.0109572. eCollection 2014.

DOI:10.1371/journal.pone.0109572
PMID:25329931
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4203730/
Abstract

Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (γ), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (Dicentrarchus labrax L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities. Despite the wide range of UV doses tested (from 7.2 to 720 mJ x cm(-2)), only one dose (60 mJ x cm(-2) x min(-1) with 1 min irradiation) resulted in a small percentage (14%) of haploid larvae at hatching in the initial trials as verified by flow cytometry. Microsatellite marker analyses of three further batches of larvae produced by using this UV treatment showed a majority of larvae with variable levels of paternal and maternal contributions and only one larva displaying pure paternal inheritance. The results are discussed also in the context of an assessment of the UV-absorbance characteristics of egg extracts in this species that revealed the presence of gadusol, a compound structurally related to mycosporine-like amino acids (MAAs) with known UV-screening properties.

摘要

雄核发育是一种单亲繁殖形式，导致后代仅继承父本染色体组。在许多淡水物种中，雄核发育已取得不同程度的成功，可通过对经γ射线、X射线或紫外线照射的遗传失活卵进行人工受精（单倍体雄核发育），以及通过压力或热休克抑制有丝分裂来恢复二倍体来实现。利用不同的紫外线照射水平和持续时间组合，探索了欧洲海鲈（Dicentrarchus labrax L.）母本基因组遗传失活的条件。紫外线处理显著影响胚胎存活，并产生了广泛的发育异常。尽管测试了广泛的紫外线剂量范围（从7.2到720 mJ x cm(-2)），但在初始试验中，只有一个剂量（60 mJ x cm(-2) x min(-1)，照射1分钟）导致孵化时出现小比例（14%）的单倍体幼虫，这通过流式细胞术得到验证。对使用这种紫外线处理产生的另外三批幼虫进行微卫星标记分析，结果显示大多数幼虫具有不同水平的父本和母本贡献，只有一只幼虫表现出纯父本遗传。还结合对该物种卵提取物紫外线吸收特性的评估对结果进行了讨论，该评估揭示了gadusol的存在，gadusol是一种与具有已知紫外线屏蔽特性的类菌孢素氨基酸（MAAs）结构相关的化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/a3204133c4cb/pone.0109572.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/ae677c1ec5c9/pone.0109572.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/5237225942b9/pone.0109572.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/55cfa918cb0e/pone.0109572.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/3aec02fe7ac7/pone.0109572.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/a3204133c4cb/pone.0109572.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/ae677c1ec5c9/pone.0109572.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/5237225942b9/pone.0109572.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/55cfa918cb0e/pone.0109572.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/3aec02fe7ac7/pone.0109572.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d35/4203730/a3204133c4cb/pone.0109572.g005.jpg

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1
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2
Use of eggs derived from the interspecific charr hybrids to induce androgenetic development of the brook charr (Salvelinus fontinalis Mitchill 1814).利用种间杂交红点鲑的卵诱导溪红点鲑（Salvelinus fontinalis Mitchill 1814）的雄核发育。
Reprod Domest Anim. 2014 Apr;49(2):191-6. doi: 10.1111/rda.12248. Epub 2013 Nov 6.
3
Induction of diploid androgenetic and mitotic gynogenetic Nile tilapia (Oreochromis niloticus L.).
BMC Genomics. 2017 Jun 7;18(1):449. doi: 10.1186/s12864-017-3826-z.
4
De novo synthesis of a sunscreen compound in vertebrates.脊椎动物中防晒化合物的从头合成。
Elife. 2015 May 12;4:e05919. doi: 10.7554/eLife.05919.
诱导二倍体雄核发育和有丝分裂雌核发育尼罗罗非鱼（Oreochromis niloticus L.）。
Theor Appl Genet. 1995 Feb;90(2):205-10. doi: 10.1007/BF00222203.
4
Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish.冷休克消除受精卵中的雌核，以诱导泥鳅（Misgurnus anguillicaudatus）的雄核发育，泥鳅是一种硬骨鱼。
BMC Biotechnol. 2011 Nov 29;11:116. doi: 10.1186/1472-6750-11-116.
5
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Ontogenez. 2011 Mar-Apr;42(2):133-45.
6
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Photochem Photobiol Sci. 2011 Jan;10(1):133-42. doi: 10.1039/c0pp00250j. Epub 2010 Nov 12.
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J Photochem Photobiol B. 2007 Nov 12;89(1):29-35. doi: 10.1016/j.jphotobiol.2007.07.006. Epub 2007 Aug 7.