Ye Feng, Yue Yafei, Li Shuhong, Chen Tianyan, Bai Guiqin, Liu Min, Zhang Shulin
Department of Infectious Disease, The First Hospital of Xi'an Jiaotong University, Xi'an, China.
Am J Obstet Gynecol. 2006 Feb;194(2):387-92. doi: 10.1016/j.ajog.2005.07.011.
The purpose of this study was to investigate the presence and distribution of Hepatitis B virus antigen (HBVAg) and Hepatitis B virus deoxyribonucleic acid (HBVDNA) in ovary with chronic hepatitis B virus.
The immunohistochemistry method and in situ hybridization method were used to detect the hepatitis B surface antigen (HBsAg), anti-hepatitis B core antibody (HBcAg), and HBVDNA in ovary of patients with chronic hepatitis B infection.
HBsAg presented in the ovum-granular cell and interstitial cell of ovary. HBsAg located in the cytomembrane and cytoplasm. HBcAg presented in the ovum-granular cell-interstitial cell and endothelium cell of interstitial blood vessel of ovary, and the HBcAg may present in the ovum at different stage. HBcAg was situated at the cytomembrane, cytoplasm, and nucleus. HBVDNA was detected in the ovum at different stage as HBcAg and in granular cell, interstitial cell of ovary. In the same ovary tissue, HBVDNA was positive in some ova, but it was weakly positive or negative in other ova. HBVDNA distributed mainly in nucleus, but also can be detected in the cytoplasm.
HBV could infect the ovum at different stage and replicate in it. This may be an important mechanism of HBV vertical transmission.
本研究旨在调查慢性乙型肝炎病毒感染患者卵巢中乙型肝炎病毒抗原（HBVAg）和乙型肝炎病毒脱氧核糖核酸（HBVDNA）的存在情况及分布。
采用免疫组织化学方法和原位杂交方法检测慢性乙型肝炎感染患者卵巢中的乙型肝炎表面抗原（HBsAg）、抗乙型肝炎核心抗体（HBcAg）和HBVDNA。
HBsAg存在于卵巢的卵母细胞-颗粒细胞和间质细胞中。HBsAg位于细胞膜和细胞质中。HBcAg存在于卵巢的卵母细胞-颗粒细胞-间质细胞和间质血管内皮细胞中，且HBcAg在不同阶段的卵母细胞中均可出现。HBcAg位于细胞膜、细胞质和细胞核中。HBVDNA在不同阶段的卵母细胞中以及卵巢颗粒细胞、间质细胞中均有检测到，与HBcAg情况一致。在同一卵巢组织中，HBVDNA在部分卵母细胞中呈阳性，但在其他卵母细胞中呈弱阳性或阴性。HBVDNA主要分布于细胞核中，但在细胞质中也可检测到。
HBV可在不同阶段感染卵母细胞并在其中复制。这可能是HBV垂直传播的重要机制。
