Ulker Bekir, Li Yong, Rosso Mario G, Logemann Elke, Somssich Imre E, Weisshaar Bernd
Max Planck Institute for Plant Breeding Research, Department of Plant Microbe Interactions, Carl-von-Linne-Weg 10, D-50829 Cologne, Germany.
Nat Biotechnol. 2008 Sep;26(9):1015-7. doi: 10.1038/nbt.1491.
Besides the well-documented integration of DNA flanked by the transfer DNA borders, occasional insertion of fragments from the tumor-inducing plasmid into plant genomes has also been reported during Agrobacterium tumefaciens-mediated transformation. We demonstrate that large (up to approximately 18 kb) gene-bearing fragments of Agrobacterium chromosomal DNA (AchrDNA) can be integrated into Arabidopsis thaliana genomic DNA during transformation. One in every 250 transgenic plants may carry AchrDNA fragments. This has implications for horizontal gene transfer and indicates a need for greater scrutiny of transgenic plants for undesired bacterial DNA.
除了有充分记录的、位于转移DNA边界两侧的DNA整合外，在根癌农杆菌介导的转化过程中，也有报道称肿瘤诱导质粒的片段偶尔会插入植物基因组。我们证明，在转化过程中，根癌农杆菌染色体DNA（AchrDNA）的大片段（高达约18 kb）携带基因的片段可以整合到拟南芥基因组DNA中。每250株转基因植物中就有一株可能携带AchrDNA片段。这对水平基因转移有影响，并表明需要对转基因植物中不需要的细菌DNA进行更严格的审查。
