Wu Minyi, Chen Ao, Li Xiaomeng, Li Xiaoyun, Hou Xingliang, Liu Xu
Guangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
University of the Chinese Academy of Sciences, Beijing, China.
Adv Biotechnol (Singap). 2024 Sep 26;2(4):34. doi: 10.1007/s44307-024-00041-9.
Plant genetic transformation is a pivotal and essential step in modifying important agronomic traits using biotechnological tools, which primarily depend on the efficacy of transgene delivery and the plant regeneration system. Over the years, advancements in the development of delivery methods and regeneration systems have contributed to plant engineering and molecular breeding. Recent studies have demonstrated that the efficiency of plant transformation can be improved by simultaneously delivering meristem-developmental regulators, utilizing virus-mediated gene editing, and executing non-sterile in planta manipulations. Efficient genetic delivery and non-tissue culture regeneration systems are gradually being developed. This review summarizes diverse delivery strategies and in planta regeneration techniques aimed at improving the efficiency of plant genetic transformation. We also emphasize the integration and utilization of these emerging transgenic approaches for expediting future crop engineering.
植物遗传转化是利用生物技术工具改良重要农艺性状的关键且必不可少的步骤，这主要取决于转基因导入的效率和植物再生系统。多年来，导入方法和再生系统的发展进步推动了植物工程和分子育种。最近的研究表明，通过同时导入分生组织发育调节因子、利用病毒介导的基因编辑以及进行非无菌的体内操作，可以提高植物转化效率。高效的遗传导入和非组织培养再生系统正在逐步开发。本综述总结了旨在提高植物遗传转化效率的多种导入策略和体内再生技术。我们还强调了这些新兴转基因方法的整合与利用，以加速未来的作物工程。
