Drewa Tomasz, Młodzik-Danielewicz Natalia, Tyrakowski Tomasz, Wolski Zbigniew, Pokrywka Lukasz, Kaczorowski Piotr, Krawczyk Agnieszka
Department of Tissue Engineering, Chair of Medical Biology, L. Rydygier Medical College Bydgoszcz, N. Copernicus University, Toruń, Poland.
Acta Pol Pharm. 2005 Sep-Oct;62(5):399-403.
Unmyelinated C-fibers endings lye beneath the epithelial layer and release neuropeptides which regulate baseline transepithelial potential difference (PD) and changes of transepithelial potential difference during mechanical stimulation (dPD). Ambroxol can suppress reflexes such as the cough or the corneal reflex which are connected to stimulation of C fibre endings. The study aimed to estimate the influence of ambroxol (ABX) and capsaicin (CAPSA) on PD and dPD in isolated rabbit bladder. The experiments were carried out on 26 bladder specimens of. 2 cm2 surface area each, obtained from 13 rabbits. Ussing apparatus was used. Procedure used for stimulation of sensory receptor involved directing stream onto epithelium. A stimulus lasted 30s, ejecting the 2.5 ml fluid. Amiloride and bumetanide were used to estimate of ionic currents. Each significant reaction was repeated at least 10 times on various specimens. PD ranged between 5 and 10 mV in different experimental conditions. Mechanical stimulation of isolated bladder wall caused increasing dPD about 2 mV defined as hyperpolarization. Application of ABX to the stimulation fluid decreased the hyperpolarization in comparison with control stimulation under conditions of inhibited chloride ion transport. Application of CAPSA to the stimulation fluid decreased the hyperpolarization in comparison with control stimulation under conditions of inhibited sodium ion transport. ABX and CAPSA had no influence on PD after mechanical stimulation. ABX as well as CAPSA influence on transepithelial ion transport pathways in bladder epithelium is dependent on sensory stimulation. ABX can be suspected to suppress bladder contractions.
无髓鞘C纤维末梢位于上皮层下方，释放神经肽，这些神经肽调节基线跨上皮电位差（PD）以及机械刺激期间跨上皮电位差的变化（dPD）。氨溴索可抑制与C纤维末梢刺激相关的反射，如咳嗽反射或角膜反射。本研究旨在评估氨溴索（ABX）和辣椒素（CAPSA）对离体兔膀胱PD和dPD的影响。实验在26个膀胱标本上进行。每个标本的表面积为2平方厘米，取自13只兔子。使用了Ussing装置。刺激感觉受体的程序包括将液体流导向上皮。刺激持续30秒，注入2.5毫升液体。用氨氯地平和布美他尼评估离子电流。每个显著反应在不同标本上至少重复10次。在不同实验条件下，PD在5至10毫伏之间。对离体膀胱壁的机械刺激导致dPD增加约2毫伏，定义为超极化。在氯离子转运受抑制的条件下，将ABX应用于刺激液中，与对照刺激相比，超极化降低。在钠离子转运受抑制的条件下，将CAPSA应用于刺激液中，与对照刺激相比，超极化降低。机械刺激后，ABX和CAPSA对PD没有影响。ABX以及CAPSA对膀胱上皮跨上皮离子转运途径的影响取决于感觉刺激。可以怀疑ABX能抑制膀胱收缩。
