Tyrakowski T, Greczko I, Sedlaczek A, Banach B, Bartłomwicz M, Wojciechowska M, Binkowska I, Chojnacka H
Department of Pathophysiology, Pomeranian University School of Medicine, Szczecin, Poland.
Pol J Pharmacol. 1998 Jan-Feb;50(1):31-8.
Isolated tracheal wall was studied by the Ussing method during selective inhibition of single, electrogenic, transepithelial ion transport pathways with either amiloride (for Na+) or bumetanide (for Cl-). It was shown that ambroxol influences the Na+ transepithelial transport pathway which is partly responsible for the hyperpolarization after mechanical stimulation of the mucosal surface of tracheal wall. Ambroxol augments the Na+ current during the presence of bumetanide in the experimental system, but diminishes it during the washing of amiloride from the measuring chamber. These bidirectional effects of ambroxol provide indirect evidence that the drug is a partial agonist for the memberance receptors which form part of the intrawall control system responsible for integration of airwall function with the transepithelial ion transport.
采用乌斯法，在分别用氨氯吡脒（针对Na+）或布美他尼（针对Cl-）选择性抑制单一的、电生性的跨上皮离子转运途径时，对分离的气管壁进行了研究。结果表明，氨溴索影响Na+跨上皮转运途径，该途径在机械刺激气管壁黏膜表面后引起的超极化中起部分作用。在实验系统中存在布美他尼的情况下，氨溴索会增强Na+电流，但在从测量室冲洗氨氯吡脒的过程中，氨溴索会使其减弱。氨溴索的这些双向作用间接证明，该药物是膜受体的部分激动剂，这些膜受体是壁内控制系统的一部分，负责气壁功能与跨上皮离子转运的整合。
