Piskorska Elzbieta, Hołyńska-Iwan Iga, Kaczorowski Piotr, Soczywko-Ciudzińska Julita, Wiciński Michał, Lampka Magdalena, Smuszkiewicz Piotr, Tyrakowski Tomasz
Departament of Pathobiochemistry and Clinical Chemistry, Nicolaus Copernicus University, Collegium Medicum, Bydgoszcz, Poland.
Int J Occup Med Environ Health. 2009;22(2):125-34. doi: 10.2478/v10001-009-0014-7.
Toluene diisocyanate (TDI) due to its widespread use in industry is one of the most common and well-known causes of occupational asthma and Reactive Airways Dysfunction Syndrome (RADS). In this study the impact of TDI on the electrophysiological properties of the airways wall, particularly on the mechanisms of absorption of sodium ions and chloride ions secretion was evaluated.
Isolated rabbit tracheal wall (from outbred stock animals) was mounted in an apparatus for electrophysiological experiments by means of Ussing method and was mechanically stimulated by the jet flux of specified fluid directed onto the mucosal surface of the tissue from a peristaltic pump. The measured parameters were: transepithelial potential difference under control conditions (PD, mV), after mechanical stimulation (dPD or physiological reaction of hyperpolarization, mV) and electric resistance (R, Omega cm2). When TDI (0.035 mM) was added to stimulation fluid, only the immediate reaction was identified and when it was added to incubation fluid and other experimental fluids, the late (post-incubation) reaction was determined. The experiments involving the inhibition of Na+ by amiloride and Cl- by bumetanide were also performed.
A series of functional tests for 72 pieces of tracheal wall from 36 animals were performed. It has been shown that short-term exposure to TDI significantly changed the course of reactions to mechanical stimulation. Also after incubation in the presence of TDI, the reactions to mechanical stimulation were changed in relation to control conditions.
The immediate reaction of the isolated rabbit tracheal wall after exposure to TDI depends on the duration of exposure and on the physiological condition of the tissue in respect of sodium and chloride ion transport.
甲苯二异氰酸酯（TDI）因其在工业中的广泛应用，是职业性哮喘和反应性气道功能障碍综合征（RADS）最常见且广为人知的病因之一。本研究评估了TDI对气道壁电生理特性的影响，特别是对钠离子吸收和氯离子分泌机制的影响。
采用Ussing方法将分离的兔气管壁（来自远交系动物）安装在电生理实验装置中，并通过蠕动泵将特定流体的喷射流导向组织的粘膜表面进行机械刺激。测量的参数有：对照条件下的跨上皮电位差（PD，mV）、机械刺激后的（dPD或超极化的生理反应，mV）和电阻（R，Ω·cm²）。当将TDI（0.035 mM）添加到刺激液中时，仅识别出即时反应；当将其添加到孵育液和其他实验液中时，测定延迟（孵育后）反应。还进行了用氨氯吡脒抑制Na⁺和用布美他尼抑制Cl⁻的实验。
对来自36只动物的72片气管壁进行了一系列功能测试。结果表明，短期接触TDI显著改变了对机械刺激的反应过程。在TDI存在下孵育后，对机械刺激的反应相对于对照条件也发生了变化。
分离的兔气管壁在接触TDI后的即时反应取决于暴露持续时间以及组织在钠离子和氯离子转运方面的生理状态。
