Sorsa Timo, Alassiri Saeed, Grigoriadis Andreas, Räisänen Ismo T, Pärnänen Pirjo, Nwhator Solomon O, Gieselmann Dirk-Rolf, Sakellari Dimitra
Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki and Helsinki University Hospital, P.O. Box 63 (Haartmaninkatu 8) FI-00014, 00100 Helsinki, Finland.
Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Diagnostics (Basel). 2020 Jan 22;10(2):61. doi: 10.3390/diagnostics10020061.
The aim of this study was to investigate the utility of incorporating active matrix metalloproteinase-8 (aMMP-8) as a biomarker into the new periodontitis classification system (stage/grade) presented in 2018. This study included 150 Greek adults aged 25-78, of whom 74 were men and 76 women. Participants were tested with an aMMP-8 point-of-care mouthrinse test, after which a full-mouth clinical examination was performed to assess their periodontal and oral health. The aMMP-8 levels in mouthrinse were significantly lower among healthy patients compared with patients in more severe periodontitis stages and grades (Kruskal-Wallis test and Dunn-Bonferroni test for pairwise post-hoc comparisons; < 0.01 and < 0.05, respectively). Furthermore, aMMP-8 levels were less correlated with plaque levels than bleeding on probing (BOP) (Spearman's rho = 0.269, < 0.001; Spearman's rho = 0.586, < 0.001); respectively). Thus, aMMP-8 was more robust to the confounding effects of oral hygiene than traditional periodontal parameter bleeding on probing. The aMMP-8 point-of-care mouthrinse test can be utilized as an adjunctive and preventive diagnostic tool to identify periodontal disease, classified by stage and grade, and ongoing periodontal breakdown chairside in clinical practice in only 5 min. Overall, integrating aMMP-8 into the new periodontitis classification system seems beneficial.
本研究的目的是调查将活性基质金属蛋白酶-8（aMMP-8）作为生物标志物纳入2018年提出的新的牙周炎分类系统（分期/分级）的效用。本研究纳入了150名年龄在25至78岁之间的希腊成年人，其中74名男性，76名女性。参与者接受了aMMP-8即时口腔冲洗测试，之后进行了全口临床检查以评估他们的牙周和口腔健康状况。与处于更严重牙周炎分期和分级的患者相比，健康患者口腔冲洗液中的aMMP-8水平显著更低（采用Kruskal-Wallis检验和Dunn-Bonferroni检验进行两两事后比较；P值分别<0.01和<0.05）。此外，与探诊出血（BOP）相比，aMMP-8水平与菌斑水平的相关性更低（Spearman相关系数分别为0.269，P<0.001；Spearman相关系数为0.586，P<0.001）。因此，与传统的牙周参数探诊出血相比，aMMP-8对口腔卫生的混杂影响更具抗性。aMMP-8即时口腔冲洗测试可作为一种辅助和预防性诊断工具，在临床实践中仅需5分钟即可在椅旁识别按分期和分级分类的牙周疾病以及正在发生的牙周组织破坏。总体而言，将aMMP-8纳入新的牙周炎分类系统似乎是有益的。
