Division of Regenerative Dentistry and Periodontology, University Clinics of Dental Medicine, University of Geneva, Geneva, Switzerland.
Diabetology Unit, University Hospitals of Geneva, Geneva, Switzerland.
Clin Exp Dent Res. 2021 Apr;7(2):170-178. doi: 10.1002/cre2.351. Epub 2020 Dec 24.
The aim of the study is to compare the levels of Gingival Crevicular Fluid (GCF) interleukin 8 (IL-8), matrix metalloproteinase 8 (MMP-8) and advanced glycated-end products (AGEs) in a cohort of type 1 diabetic (T1D) subjects and healthy controls.
GCF samples and periodontal examination were assessed in 50 subjects with T1D (30 males and 20 females; mean age: 35.2 years) recruited from the Diabetology Unit of the Geneva University Hospitals and in 50 control subjects matched for gender, age and smoking status. Samples were assessed for IL-8 and MMP-8 using a bead array multianalyte detection system and for AGEs the ELISA. The two groups were compared using the Wilcoxon signed rank test.
The mean HbA1c differed significantly between the groups (8.3% for the T1D group vs. 5.2% for the control group, p < 0.001). T1D subjects had significantly more plaque and gingival inflammation and presented more sites with bleeding on probing compared to the controls. The GCF levels of IL-8, MMP-8 and AGEs did not differ significantly between the groups. Further analysis of the GCF markers in younger (<40 years) and older (≥40 years) cohorts, revealed no significant differences between younger diabetics and controls or between older diabetics and controls. When the groups were divided according to their glycemic status (HbA1c 6.1-8, and > 8%), again no significant differences could be identified for any of the biochemical markers.
T1D subjects, particularly the younger ones, exhibited more inflammation compared to the matched healthy controls. Results on the GCF expression of IL-8, MMP-8 and AGEs did not differ between the groups. The diabetic population of our cohort was for the most part fairly-controlled, with little if any complications and with presence of only mild type of periodontal disease, as 68% had gingivitis.
本研究旨在比较 1 型糖尿病（T1D）患者和健康对照者龈沟液（GCF）中白细胞介素 8（IL-8）、基质金属蛋白酶 8（MMP-8）和晚期糖基化终产物（AGEs）的水平。
从日内瓦大学医院糖尿病科招募了 50 名 T1D 患者（30 名男性和 20 名女性；平均年龄：35.2 岁）和 50 名性别、年龄和吸烟状况相匹配的健康对照者，评估 GCF 样本和牙周检查。使用 bead array 多分析物检测系统评估 IL-8 和 MMP-8，使用 ELISA 评估 AGEs。使用 Wilcoxon 符号秩检验比较两组。
两组间的平均 HbA1c 差异有统计学意义（T1D 组为 8.3%，对照组为 5.2%，p<0.001）。T1D 患者的菌斑和牙龈炎症明显多于对照组，探诊出血的位点也明显多于对照组。两组间 GCF 中 IL-8、MMP-8 和 AGEs 的水平无显著差异。对年龄较小（<40 岁）和较大（≥40 岁）两组的 GCF 标志物进行进一步分析，发现年轻糖尿病患者与对照组之间或老年糖尿病患者与对照组之间无显著差异。当根据血糖状况（HbA1c 6.1-8 和>8%）将两组进行分组时，也无法确定任何生化标志物存在显著差异。
与匹配的健康对照组相比，T1D 患者，尤其是年轻患者，表现出更多的炎症。两组间 GCF 中 IL-8、MMP-8 和 AGEs 的表达无差异。我们队列中的糖尿病患者大多血糖控制较好，几乎没有并发症，且仅存在轻度牙周病，68%的患者患有牙龈炎。
