Chong Y C, Duffus W P, Field H J, Gray D A, Awan A R, O'Brien M A, Lunn D P
Department of Clinical Veterinary Medicine, Cambridge, UK.
Equine Vet J. 1991 Mar;23(2):111-5. doi: 10.1111/j.2042-3306.1991.tb02733.x.
Over a period of two years, a total of 22 full term foals from Welsh Mountain pony mares were raised in conditions that were free from infection by Equid herpesvirus (EHV-1/4). Parturition dates were predicted by monitoring colostrum electrolytes, and the mares allowed to foal naturally under supervision or following induction with intravenous oxytocin. Immediately following birth, foals were separated from their dams and transferred to a specially built, positive pressure isolation unit. They were given antibiotic prophylaxis and fed bovine colostrum during the first 24 h, and then mare's milk replacer until weaned. Out of 22 specific pathogen free (SPF) foals one that had not been given antibiotic prophylaxis died of an E. coli septicaemia aged eight days. Two foals developed a streptococcal upper respiratory tract infection, which responded to antibiotic therapy and did not spread to the rest of the herd. A self limiting upper respiratory tract infection was seen in a fourth foal and mild diarrhoea was observed in six foals. Physical development in all SPF foals appeared normal and behavioural patterns resembled those of conventional handreared foals. Newborn foals were held in a separate quarantine area, within the isolation unit, and checked extensively for evidence of EHV-1/4 infection, before being transferred to the main holding unit. Periodic checks were then made for EHV-1/4 over a period ranging from 2 to 4 months; none of the SPF foals showed evidence of infection with EHV-1/4 in terms of clinical disease, virus isolation, sero-conversion or specific lymphocyte transformation.
在两年的时间里，共有22匹威尔士山地小马母马所生的足月马驹在无马疱疹病毒（EHV - 1/4）感染的条件下饲养。通过监测初乳电解质来预测分娩日期，母马在监督下自然分娩或静脉注射催产素诱导分娩。出生后，马驹立即与母马分离，并转移到一个专门建造的正压隔离单元。在出生后的头24小时内，给它们进行抗生素预防并喂食牛初乳，然后喂马奶替代品直至断奶。在22匹无特定病原体（SPF）的马驹中，有一匹未接受抗生素预防的马驹在8天大时死于大肠杆菌败血症。两匹马驹患上了链球菌性上呼吸道感染，经抗生素治疗后康复，且未传播至马群其他个体。第四匹马驹出现了自限性上呼吸道感染，六匹马驹出现了轻度腹泻。所有SPF马驹的身体发育看起来正常，行为模式与传统人工饲养的马驹相似。新生马驹被安置在隔离单元内的一个单独隔离区，在转移到主饲养单元之前，对其进行广泛检查以寻找EHV - 1/4感染的证据。然后在2至4个月的时间内定期检查EHV - 1/4；就临床疾病、病毒分离、血清转化或特异性淋巴细胞转化而言，没有一匹SPF马驹显示出EHV - 1/4感染的迹象。
