Foote C E, Raidal S L, Pecenpetelovska G, Wellington J E, Whalley J M
Department of Biological Sciences, Macquarie University, Sydney 2109, Australia.
Vet Immunol Immunopathol. 2006 May 15;111(1-2):97-108. doi: 10.1016/j.vetimm.2006.01.012. Epub 2006 Feb 28.
We have previously demonstrated that intramuscular inoculation of EHV-1 glycoprotein D (gD) and glycoprotein B (gB) produced by a recombinant baculovirus and formulated with the adjuvant Iscomatrix elicited virus-neutralizing antibody and gD- and gB-specific ELISA antibody in adult horses. In this study, 14 mares and their very young foals were inoculated with a combination of baculovirus-expressed EHV-1 gD and EHV-1 gB (EHV-1 gDBr) and challenged with a respiratory strain of EHV-1. Following experimental challenge, inoculated mares and foals shed virus in nasal secretions on significantly fewer occasions compared to uninoculated mares and foals. Uninoculated foals born from inoculated mares were no more protected against experimental challenge than uninoculated foals born from uninoculated mares. The results suggest that it is indeed possible to induce partial protection in very young foals through vaccination, and while the inoculation did not prevent infection, it did reduce the frequency of viral shedding with the potential to thereby reduce the risk and prevalence of infection in a herd situation.
我们之前已经证明，用重组杆状病毒产生并与佐剂Iscomatrix配制的马疱疹病毒1型糖蛋白D（gD）和糖蛋白B（gB）进行肌肉接种，可在成年马中引发病毒中和抗体以及gD和gB特异性ELISA抗体。在本研究中，14匹母马及其幼驹接种了杆状病毒表达的马疱疹病毒1型gD和马疱疹病毒1型gB的组合（EHV-1 gDBr），并接受马疱疹病毒1型呼吸道毒株的攻击。实验攻击后，与未接种的母马和幼驹相比，接种的母马和幼驹在鼻分泌物中排出病毒的次数明显减少。接种母马所生的未接种幼驹，与未接种母马所生的未接种幼驹相比，在抵御实验攻击方面并没有得到更多保护。结果表明，通过疫苗接种确实有可能在非常年幼的幼驹中诱导部分保护，并且虽然接种不能预防感染，但它确实降低了病毒排出的频率，从而有可能降低群体感染的风险和流行率。
