在类风湿性关节炎或骨关节炎患者的滑膜组织和血液样本中未检测到人类逆转录病毒5前病毒DNA。

Lack of detection of human retrovirus-5 proviral DNA in synovial tissue and blood specimens from individuals with rheumatoid arthritis or osteoarthritis.

作者信息

Piper Kerryl E, Hanssen Arlen D, Lewallen David G, Matteson Eric L, Osmon Douglas R, Duffy Mary C, Hagan Rochelle A, Steckelberg James M, Patel Robin

机构信息

Division of Infectious Diseases, Dept. of Medicine, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.

出版信息

Arthritis Rheum. 2006 Feb 15;55(1):123-5. doi: 10.1002/art.21690.

DOI:10.1002/art.21690

PMID:16463423

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1464419/

Abstract

OBJECTIVE

Prior studies have suggested an association of human retrovirus 5 with rheumatoid arthritis. The purpose of this study was to determine if human retrovirus-5 proviral DNA is present in synovial tissue and blood specimens from patients with rheumatoid arthritis or osteoarthritis, or those without joint disease.

METHODS

Synovial tissue and whole blood from 75 patients with rheumatoid arthritis, 75 patients with osteoarthritis, and 50 patients without a primary arthritis diagnosis were assayed by real-time quantitative polymerase chain reaction (PCR) using primers that amplify a 186-bp fragment of human retrovirus-5 proviral DNA.

RESULTS

A total of 200 tissue specimens, 200 mononuclear cells, and 196 of 200 granulocyte specimens tested negative for human retrovirus-5 proviral DNA. No association between human retrovirus 5 and rheumatoid arthritis or osteoarthritis (P = 0.516) was identified. Granulocyte specimens from 4 patients, 2 with rheumatoid arthritis and 2 with osteoarthritis, yielded a low positive human retrovirus-5 proviral DNA signal (83-1,365 copies of human retrovirus-5 proviral DNA/ml blood).

CONCLUSION

Contrary to prior reports, we did not find an association between human retrovirus 5 and rheumatoid arthritis or osteoarthritis using a real-time PCR assay. Our findings are consistent with the recent finding that human retrovirus 5 is actually rabbit endogenous retrovirus H.

摘要

目的

先前的研究表明人类逆转录病毒5与类风湿关节炎有关联。本研究的目的是确定类风湿关节炎患者、骨关节炎患者或无关节疾病患者的滑膜组织和血液标本中是否存在人类逆转录病毒5前病毒DNA。

方法

对75例类风湿关节炎患者、75例骨关节炎患者和50例无原发性关节炎诊断的患者的滑膜组织和全血，使用能扩增人类逆转录病毒5前病毒DNA 186碱基对片段的引物，通过实时定量聚合酶链反应（PCR）进行检测。

结果

总共200份组织标本、200份单核细胞标本以及200份粒细胞标本中的196份，人类逆转录病毒5前病毒DNA检测呈阴性。未发现人类逆转录病毒5与类风湿关节炎或骨关节炎之间存在关联（P = 0.516）。4例患者的粒细胞标本检测出人类逆转录病毒5前病毒DNA呈低阳性信号（每毫升血液中人类逆转录病毒5前病毒DNA有83 - 1365拷贝），其中2例为类风湿关节炎患者，2例为骨关节炎患者。