Li Yiqing, Zhang Yong, Zheng Qichang
Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2005;25(5):552-4. doi: 10.1007/BF02896015.
In order to study the expression of transformation suppressor gene RECK and MMP-9 in hilar cholangiocarcinomas and its clinical significance, and explore the roles of RECK gene in metastasis and invasion of hilar cholangiocarcinoma, the expression levels of RECK, and MMP-9 mRNA were detected by using reverse transcription-polymerase reaction in 42 paraffin-embedded samples of hilar cholangiocarcinomas and 10 samples of benign bile duct diseases. The results showed that in hilar cholangiocarcinoma tissues, the expression of RECK gene was 0.235 +/- 0.062, significantly lower than in normal bile duct tissues (0.533 +/- 0.024, P < 0.05). In hilar cholangiocarcinoma tissues, the expression of MMP-9 (0.528 +/- 0.039) was significantly higher than in the normal tissues (0.311 +/- 0.032, P < 0.05). The expression of RECK gene was closely related to the intrahepatic and surrounding organs invasion (P < 0.05). It was concluded that RECK gene could inhibit the expression of MMP-9 in hilar cholangiocarcinomas and closely correlated with the biological behaviors. The abnormal expression of RECK gene might be one of the molecular mechanisms of hilar cholangiocarcinoma metastasis.
为研究转化抑制基因RECK和基质金属蛋白酶-9（MMP-9）在肝门部胆管癌中的表达及其临床意义，探讨RECK基因在肝门部胆管癌转移和侵袭中的作用，采用逆转录聚合酶反应检测42例肝门部胆管癌石蜡包埋组织及10例胆管良性疾病组织中RECK、MMP-9 mRNA的表达水平。结果显示，肝门部胆管癌组织中RECK基因表达为0.235±0.062，显著低于正常胆管组织（0.533±0.024，P<0.05）。肝门部胆管癌组织中MMP-9表达（0.528±0.039）显著高于正常组织（0.311±0.032，P<0.05）。RECK基因表达与肝内及周围器官侵犯密切相关（P<0.05）。结论：RECK基因可抑制肝门部胆管癌中MMP-9的表达，且与生物学行为密切相关。RECK基因异常表达可能是肝门部胆管癌转移的分子机制之一。
