Li Wei-Ren, Cen Ying, Li Xiao-Hong, Liao Dian-Ying
Department of Plastic and Burn Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Mar;39(2):243-6.
To study the change of xanthine oxidase in pathological scars.
The tissues of hypertrophic scar (10 cases), keloid (10 cases) and normal skin (8 cases) were collected for this project, in which the content of malonaldehyde, the activity of xanthine oxidase and total of antioxidant capacity were detected by spectrophotometric method. The expression of xanthine oxidase was evaluated by immunohistochemistry technique.
Compared with normal skin tissue, the content of malonaldehyde and xanthine oxidase activity were significantly higher in pathological scars (P < 0.05), but no significant difference was observed in the decrease of total antioxidant capacity. Differences in above-mentioned indexes all were not remarkable between hypertrophic scars and keloids. Immunohistochemical study indicated that the expressions of xanthine oxidase in the epidermal keratinocytes and dermal fibroblasts of pathological scars increased markedly in contrast with normal skin (P < 0.05).
The xanthine oxidase activity shows to be increased in pathological scars, and the xanthine oxidase expressions are enlarged in epidermal keratinocytes and dermal fibroblasts of pathological scars, which may be a reason resulting in the increase of free excessive growth of scar tissues. radical level, and may play a role in pathogenesis of excessive growth of scar tissues.
研究病理性瘢痕中黄嘌呤氧化酶的变化。
本研究收集了10例增生性瘢痕、10例瘢痕疙瘩及8例正常皮肤组织，采用分光光度法检测丙二醛含量、黄嘌呤氧化酶活性及总抗氧化能力，采用免疫组织化学技术评估黄嘌呤氧化酶的表达。
与正常皮肤组织相比，病理性瘢痕中丙二醛含量和黄嘌呤氧化酶活性显著升高（P<0.05），但总抗氧化能力降低差异无统计学意义。增生性瘢痕与瘢痕疙瘩上述指标差异均不显著。免疫组织化学研究表明，与正常皮肤相比，病理性瘢痕的表皮角质形成细胞和真皮成纤维细胞中黄嘌呤氧化酶的表达明显增加（P<0.05）。
病理性瘢痕中黄嘌呤氧化酶活性升高，且在病理性瘢痕的表皮角质形成细胞和真皮成纤维细胞中黄嘌呤氧化酶表达增强，这可能是导致瘢痕组织自由基水平升高及瘢痕组织过度生长的原因之一，可能在瘢痕组织过度生长的发病机制中起作用。
