Bernardi F, Bernardi A
Laboratoire d'Enzymologie, CNRS, Gif-sur-Yvette, France.
Mol Gen Genet. 1991 May;227(1):22-7. doi: 10.1007/BF00260701.
We have performed a detailed analysis of intra- and intermolecular endproducts of transposition of the compound transposon Tn903 and we show that, in our system, the transposition activity is almost entirely driven by one of the flanking insertion sequences, IS903L. The relatively inactive state of IS903R can be conferred on IS903L by changing the orientation of the internal Tn region. IS903L mediates the formation of the majority of adjacent deletions, insertion/inversions and cointegrates, all of which are representative of replicative transposition; only a very low level of conservative transposition can be observed. Our results are discussed in relation to those showing that Tn903 uses predominantly the conservative pathway.
我们对复合转座子Tn903转座的分子内和分子间终产物进行了详细分析，结果表明，在我们的系统中，转座活性几乎完全由侧翼插入序列之一IS903L驱动。通过改变内部Tn区域的方向，IS903R的相对无活性状态可以赋予IS903L。IS903L介导了大多数相邻缺失、插入/倒位和共整合体的形成，所有这些都是复制性转座的代表；只能观察到非常低水平的保守转座。我们将结合那些表明Tn903主要使用保守途径的结果来讨论我们的结果。
