Bernardi A, Bernardi F
Gene. 1981 Jan-Feb;13(1):103-9. doi: 10.1016/0378-1119(81)90047-0.
Large deletions occur in the hybrid plasmid formed by pSC101 and the EcoRI fragment f2 of phage lambda (redB-ori region) under well defined growth conditions (Bernardi and Bernardi, 1980). We have sequenced the novel joints of the four deletions so obtained and shown that they have one endpoint in pSC101, identical in all four cases, the other endpoint being located in four different lambda sequences. Furthermore, the nucleotide sequences of the novel joints show homologies between the conserved pSC101 sequence and the lambda sequences both conserved and deleted. The presence of an IS-type element in pSC101 is postulated; however, this element is unrelated to the 200 bp element already described in pSC101 (Ravetch et al., 1976).
在明确的生长条件下，由pSC101和噬菌体λ的EcoRI片段f2（redB - ori区域）形成的杂种质粒中会出现大片段缺失（贝尔纳迪和贝尔纳迪，1980年）。我们已对如此获得的四个缺失的新连接点进行了测序，并表明它们在pSC101中有一个端点，在所有四种情况下均相同，另一个端点位于四个不同的λ序列中。此外，新连接点的核苷酸序列显示，保守的pSC101序列与λ序列（包括保守和缺失的序列）之间存在同源性。推测pSC101中存在一个IS型元件；然而，该元件与pSC101中已描述的200 bp元件无关（拉韦奇等人，1976年）。
