Tsai Nan-Chyuan, Sue Chung-Yang
Department of Mechanical Engineering, National Cheng Kung University, Taiwan.
Biosens Bioelectron. 2006 Aug 15;22(2):313-7. doi: 10.1016/j.bios.2005.12.023. Epub 2006 Feb 7.
Being beneficial from dramatic progress in bio-chemical and micro-electro-mechanical technologies, DNA manipulation devices can be miniaturized to expedite amplification processing for virus detection. Polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR) are two typical examples of them. In this report, a micro-RT-PCR (microRT-PCR) chip is designed to quantitatively detect tumor viruses. Test sample reservoirs, RT-PCR meanders, and capillary electrophoresis are integrated on a SU-8 based monolithic chip. A high-precision temperature control module is well developed by embedding signal amplification circuits and an Intel 8051 microprocessor. The integrated system exhibits high efficacy for heat transfer, exemption of fluid flow clogging and superior sensitivity to precisely control the required reaction temperatures in all heated zones of microRT-PCR unit. Intensive simulations and experiments are presented to verify the validity of the fabricated bio-chip.
受益于生化和微机电技术的巨大进步，DNA 操纵设备可以小型化，以加快病毒检测的扩增处理。聚合酶链反应（PCR）和逆转录聚合酶链反应（RT-PCR）就是其中两个典型例子。在本报告中，设计了一种微 RT-PCR（microRT-PCR）芯片用于定量检测肿瘤病毒。测试样品储液器、RT-PCR 微通道和毛细管电泳集成在一个基于 SU-8 的单片芯片上。通过嵌入信号放大电路和英特尔 8051 微处理器，开发出了一个高精度温度控制模块。该集成系统具有高效的热传递性能，可避免流体流动堵塞，并且在精确控制 microRT-PCR 单元所有加热区域所需反应温度方面具有卓越的灵敏度。文中展示了大量模拟和实验，以验证所制造生物芯片的有效性。
