用于细胞生物学和分析的由热塑性塑料制成的微流控装置的进展。
Advances in microfluidic devices made from thermoplastics used in cell biology and analyses.
作者信息
Gencturk Elif, Mutlu Senol, Ulgen Kutlu O
机构信息
Department of Chemical Engineering, Biosystems Engineering Laboratory, Bogazici University, 34342 Istanbul, Turkey.
Department of Electrical and Electronics Engineering, BUMEMS Laboratory, Bogazici University, 34342 Istanbul, Turkey.
出版信息
Biomicrofluidics. 2017 Oct 24;11(5):051502. doi: 10.1063/1.4998604. eCollection 2017 Sep.
Silicon and glass were the main fabrication materials of microfluidic devices, however, plastics are on the rise in the past few years. Thermoplastic materials have recently been used to fabricate microfluidic platforms to perform experiments on cellular studies or environmental monitoring, with low cost disposable devices. This review describes the present state of the development and applications of microfluidic systems used in cell biology and analyses since the year 2000. Cultivation, separation/isolation, detection and analysis, and reaction studies are extensively discussed, considering only microorganisms (bacteria, yeast, fungi, zebra fish, etc.) and mammalian cell related studies in the microfluidic platforms. The advantages/disadvantages, fabrication methods, dimensions, and the purpose of creating the desired system are explained in detail. An important conclusion of this review is that these microfluidic platforms are still open for research and development, and solutions need to be found for each case separately.
硅和玻璃是微流控装置的主要制造材料，然而，在过去几年中塑料的使用呈上升趋势。热塑性材料最近已被用于制造微流控平台，以进行细胞研究或环境监测实验，这些设备成本低且可一次性使用。本综述描述了自2000年以来用于细胞生物学的微流控系统的发展现状和应用，并进行了分析。广泛讨论了培养、分离/隔离、检测与分析以及反应研究，这里仅考虑微流控平台中与微生物（细菌、酵母、真菌、斑马鱼等）和哺乳动物细胞相关的研究。详细解释了这些系统的优缺点、制造方法、尺寸以及创建所需系统的目的。本综述的一个重要结论是，这些微流控平台仍有待研发，需要针对每种情况分别找到解决方案。
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