Derka S, Vairaktaris E, Papakosta V, Vassiliou S, Acil Y, Vylliotis A, Spyridonidou S, Lazaris A C, Mourouzis C, Kokkori A, Moulavasili P, Perrea D, Donta I, Yapijakis C, Patsouris E
Department of Oral and Maxillofacial Surgery, University of Athens Medical School, Greece.
Oral Oncol. 2006 May;42(5):540-50. doi: 10.1016/j.oraloncology.2005.10.008. Epub 2006 Feb 7.
Markers of cell proliferation (Ki-67 antigen) and apoptosis (Bax, Bcl-2) were studied in an experimental system of induced oral carcinogenesis in Syrian golden hamsters. Thirty-seven animals were divided into one control group and three experimental groups, which were treated with a carcinogen and sacrificed at 10, 14 and 19 weeks after treatment. The histological status of the lesions in the three experimental groups corresponded well with tumour advancement (from oral mucosal dysplasia to moderately differentiated squamous cell carcinoma). Tumour sections were studied using monoclonal antibodies against Bax, Bcl-2 and Ki-67 proteins. Pro-apoptotic Bax expression maintained high levels during all stages of oral carcinogenesis. Anti-apoptotic Bcl-2 expression decreased significantly in dysplastic and early invasion lesions and consequently increased almost to normal tissue level in consequent stages. Finally, Ki-67 expression increased sharply in initial stages of oral carcinogenesis, but significantly decreased in later stages.
在叙利亚金黄地鼠诱导口腔癌发生的实验系统中，研究了细胞增殖标志物（Ki-67抗原）和细胞凋亡标志物（Bax、Bcl-2）。37只动物被分为1个对照组和3个实验组，实验组用致癌物处理，并在处理后10周、14周和19周处死。三个实验组病变的组织学状态与肿瘤进展（从口腔黏膜发育异常到中度分化鳞状细胞癌）高度吻合。使用针对Bax、Bcl-2和Ki-67蛋白的单克隆抗体对肿瘤切片进行研究。促凋亡的Bax表达在口腔癌发生的所有阶段均维持在高水平。抗凋亡的Bcl-2表达在发育异常和早期浸润性病变中显著降低，随后在后续阶段几乎增加到正常组织水平。最后，Ki-67表达在口腔癌发生的初始阶段急剧增加，但在后期显著降低。
