Vairaktari Efstathia, Schramm Alexander, Vairaktari Georgia, Derka Spyridoula, Wilde Frank, Sakkas Andreas, Yapijakis Christos, Kouri Maria, Balakas Athanasios, Lazaris Andreas, Ebeling Marcel, Vassiliou Stavros
Department of Oral and Maxillofacial Surgery, University General Hospital Attikon, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Department of Oral and Maxillofacial Surgery, University Hospital Ulm, Albert-Einstein-Allee 10, 89081 Ulm, Germany.
J Pers Med. 2024 Jul 25;14(8):790. doi: 10.3390/jpm14080790.
The primary aim of this study was to evaluate the activation of the PERP and Akt oncogenes in the induction of skin cancer in FVB/N mice by a stepwise chemical process. Forty four-week-old female FVB/N mice were randomly divided into a control group (n = 8) and two experimental groups (group A: n = 16, group B: n = 16). In the study, the groups were subjected to a two-stage carcinogenesis procedure. This consisted of an initial application of 97.4 nmol DMBA to shaved skin on the back, followed by applications of 32.4 nmol TPA after thirteen weeks for group A and after twenty weeks for group B. The control group received no treatment. Skin conditions were monitored weekly for tumor development. At the end of the experiment, the animals were euthanized for further tissue sampling. Examination of the skin lesions in the experimental groups showed a correlation with tumor progression, ranging from dysplasia to carcinoma. Tumor samples were examined both histologically and immunohistochemically. Notably, and PERP expression was higher in precancerous than in malignant tumors. The differences in expression between precancerous and benign tumors provide further evidence of a role for PERP and Akt in the transition from benign to malignant states. Our findings underscore the critical roles of PERP and Akt in the pathogenesis of skin cancer and suggest their potential as biomarkers for early detection and targets for therapeutic intervention.
本研究的主要目的是评估在FVB/N小鼠中通过逐步化学过程诱导皮肤癌时PERP和Akt致癌基因的激活情况。将44只4周龄雌性FVB/N小鼠随机分为对照组（n = 8）和两个实验组（A组：n = 16，B组：n = 16）。在该研究中，各实验组均接受两阶段致癌程序。该程序包括首先在背部剃毛皮肤上涂抹97.4 nmol二甲基苯并蒽（DMBA），随后A组在13周后、B组在20周后涂抹32.4 nmol佛波酯（TPA）。对照组不接受任何处理。每周监测皮肤状况以观察肿瘤发展情况。实验结束时，对动物实施安乐死以进行进一步的组织取样。对实验组皮肤病变的检查显示其与肿瘤进展相关，范围从发育异常到癌。对肿瘤样本进行了组织学和免疫组织化学检查。值得注意的是，PERP在癌前肿瘤中的表达高于恶性肿瘤。癌前肿瘤与良性肿瘤之间表达的差异进一步证明了PERP和Akt在从良性状态向恶性状态转变中的作用。我们的研究结果强调了PERP和Akt在皮肤癌发病机制中的关键作用，并表明它们作为早期检测生物标志物和治疗干预靶点的潜力。
