Moennig V, Leder L, Greiser-Wilke I, Frey H R, Liess B
Institut für Virologie, Tierärztlichen Hochschule, Hannover.
Tierarztl Prax. 1991 Feb;19(1):35-8.
An enzyme-linked immunosorbent assay (ELISA), using the nonstructural protein p125/80 of the bovine viral diarrhoea (BVD) virus as antigen, was used for screening BVD-specific antibodies in 468 bovine sera. The results were compared with those obtained in a standard neutralization test (NT). Both tests reacted positive with 457 sera. The ELISA gave positive results with eight sera which were negative in the NT whereas one serum was positive in the NT and negative in the ELISA. Two sera could not be tested in the NT because they were toxic for the cultured cells. In addition the sera were screened in an ELISA using the equivalent heterologous nonstructural protein of hog cholera virus as antigen. The results obtained in both ELISAs showed a correlation of 94%.
采用牛病毒性腹泻（BVD）病毒的非结构蛋白p125/80作为抗原的酶联免疫吸附测定（ELISA），用于检测468份牛血清中的BVD特异性抗体。将结果与标准中和试验（NT）的结果进行比较。两种检测方法对457份血清的反应均为阳性。ELISA检测出8份血清呈阳性，而这些血清在中和试验中呈阴性；有1份血清在中和试验中呈阳性，但在ELISA检测中呈阴性。有两份血清无法进行中和试验，因为它们对培养细胞有毒性。此外，还用猪霍乱病毒的等效异源非结构蛋白作为抗原，通过ELISA对血清进行检测。两种ELISA检测结果的相关性为94%。
