Pejsak Z, Lipowski A, Truszczynski M
National Veterinary Research Institute, Department of Swine Diseases, Pulawy, Poland.
Rev Sci Tech. 1993 Sep;12(3):879-85. doi: 10.20506/rst.12.3.732.
Between 1990 and 1992, serum samples from 55,478 domestic swine were tested by enzyme-linked immunosorbent assay (ELISA) for the presence of hog cholera virus (HCV) antibodies. The amount of antibody in the sera was expressed as the mean percentage inhibition (PI). For diagnosis, the tested sera were diluted 1:2 and considered positive if the PI was less than 25%. Sera giving PI values in the range of 25-50% were retested against HCV and bovine virus diarrhoea virus (BVDV), by neutralising peroxidase-linked assay. Comparison of the serum titres obtained was used for serological diagnosis of hog cholera; the tested sera were considered negative for hog cholera if the titre for BVDV was higher than that obtained for HCV. All sera with a PI higher than 50% were considered negative for HCV and BVDV. All sera were found to be free of antibodies to HCV. BVDV antibodies were demonstrated in 0.40% of the sera tested in 1990, in 1.80% in 1991 and 1.06% in 1992.
1990年至1992年期间，采用酶联免疫吸附测定法（ELISA）对55478份家猪血清样本进行检测，以确定是否存在猪瘟病毒（HCV）抗体。血清中的抗体量以平均抑制百分比（PI）表示。诊断时，将检测的血清稀释1:2，若PI小于25%则判定为阳性。PI值在25%-50%之间的血清，通过中和过氧化物酶联试验针对HCV和牛病毒性腹泻病毒（BVDV）进行重新检测。比较所获得的血清滴度用于猪瘟的血清学诊断；若BVDV的滴度高于HCV的滴度，则检测的血清判定为猪瘟阴性。所有PI高于50%的血清均判定为HCV和BVDV抗体阴性。所有血清均未检测到HCV抗体。1990年检测的血清中，0.40%检测到BVDV抗体，1991年为1.80%，1992年为1.06%。
