一种用于血清学检测抗欧洲猪瘟病毒抗体的灵敏酶联免疫吸附测定（ELISA）
[A sensitive enzyme-linked immunosorbent assay (ELISA) for the serological detection of antibodies against the European hog cholera virus].
作者信息
Schagemann G, Greiser-Wilke I, Liess B, Moennig V
机构信息
Institut für Virologie, Tierärztlichen Hochschule, Hannover.
出版信息
Tierarztl Prax. 1991 Feb;19(1):54-7.
PMID:1646495
Abstract
An ELISA for the detection of antibodies against hog cholera virus (HCV) was developed. The HCV-specific glycoprotein gp53 served as diagnostic antigen after immobilization using a monoclonal capture antibody. Due to the higher affinity of HCV-specific antibodies to the viral gp53, sera cross reacting with bovine viral diarrhea (BVD) virus were discriminated by the slope of the titration curves.
摘要
开发了一种用于检测抗猪霍乱病毒（HCV）抗体的酶联免疫吸附测定（ELISA）。HCV特异性糖蛋白gp53在使用单克隆捕获抗体固定后用作诊断抗原。由于HCV特异性抗体对病毒gp53具有更高的亲和力，通过滴定曲线的斜率区分了与牛病毒性腹泻（BVD）病毒发生交叉反应的血清。
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