Yokoyama Yuichiro, Kuramitsu Yasuhiro, Takashima Motonari, Iizuka Norio, Terai Shuji, Oka Masaaki, Nakamura Kazuyuki, Okita Kiwamu, Sakaida Isao
Department of Gastroenterology and Hepatology, Yamaguchi University School of Medicine, Ube, Yamaguchi 755-8505, Japan.
Int J Oncol. 2006 Mar;28(3):625-31.
Many reports suggest that hepatic steatosis leads to hepatocellular carcinoma (HCC), including hepatitis C virus or non-alcoholic steatohepatitis. Proteomic study of tumor tissues from HCC patients, focusing on apolipoprotein (apo) of apoA1, apoB100 and apoE, was performed by immunoblotting. Although the significant changes of apoA1 or apoB100 could not be shown statistically, the immunoblotting showed the increase in protein level of apoE in the tumor tissues of 88% of patients without increase of apoE gene expression and serum level. These results suggest the accumulation of apoE by impaired secretion. Moreover, immunoblot analysis on two-dimensional electrophoresis showed a strong possibility that sialylated forms of apoE also were increased in tumorous tissues of HCC. ApoE level in tumorous tissues is frequently elevated and may be a good histological marker for HCC.
许多报告表明，肝脂肪变性会导致肝细胞癌（HCC），包括丙型肝炎病毒或非酒精性脂肪性肝炎。通过免疫印迹法对HCC患者肿瘤组织进行蛋白质组学研究，重点关注载脂蛋白A1（apoA1）、载脂蛋白B100（apoB100）和载脂蛋白E（apoE）。尽管从统计学上无法显示apoA1或apoB100有显著变化，但免疫印迹显示，88%患者的肿瘤组织中apoE蛋白水平升高，而apoE基因表达和血清水平并未升高。这些结果表明，apoE通过分泌受损而积累。此外，二维电泳的免疫印迹分析显示，HCC肿瘤组织中apoE的唾液酸化形式也很可能增加。肿瘤组织中的apoE水平经常升高，可能是HCC的一个良好组织学标志物。
