School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459, Singapore.
Curr Microbiol. 2010 Oct;61(4):280-4. doi: 10.1007/s00284-010-9608-3. Epub 2010 Feb 18.
Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This work aims to investigate the secreted proteins in our recently established cell-based HBV replication system, using isobaric tags for relative and absolute quantitation (iTRAQ)-coupled 2D LC-MS/MS proteomics approach. Such proteins are reflective of early events of HBV infection and thus may have potential as prognostic biomarkers for development of liver diseases.
乙型肝炎病毒（HBV）感染仍然是一个主要的健康问题，全球有超过 3.5 亿携带者。它与急性和慢性肝病有关，包括肝细胞癌（HCC）。早期发现与 HBV 相关的严重肝病对于有效治疗至关重要。本工作旨在使用等重标记相对和绝对定量（iTRAQ）联合二维 LC-MS/MS 蛋白质组学方法研究我们最近建立的基于细胞的 HBV 复制系统中的分泌蛋白。这些蛋白反映了 HBV 感染的早期事件，因此可能有作为肝病发展的预后生物标志物的潜力。
