Thompson S T, Cass K H, Stellwagen E
Proc Natl Acad Sci U S A. 1975 Feb;72(2):669-72. doi: 10.1073/pnas.72.2.669.
A procedure is described to utilize blue dextran-Sepharose as an affinity chromatographic column specific for the super-secondary structure called the dinucleotide fold, which forms the binding sites for substrates and effectors on a wide range of proteins. The procedure can be used to identify proteins, either purified or in crude cellular extracts, that possess the dinucleotide fold and to significantly improve the purification procedures for those proteins that possess the fold.
本文描述了一种利用蓝色葡聚糖-琼脂糖凝胶作为亲和色谱柱的方法，该色谱柱对一种名为二核苷酸折叠的超二级结构具有特异性，这种结构构成了多种蛋白质上底物和效应物的结合位点。该方法可用于鉴定纯化的或粗细胞提取物中具有二核苷酸折叠的蛋白质，并显著改进那些具有该折叠结构的蛋白质的纯化程序。
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