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Cadmium effects on Ichthyophthirius: evidence for metal-sequestration in fish tissues following administration of recombinant vaccines.镉对鱼波豆虫的影响：接种重组疫苗后鱼类组织中金属螯合的证据。
Parasitology. 2003;126 Suppl:S87-93. doi: 10.1017/s0031182003003731.
2
Cloning and sequencing of four new metallothionein genes from Tetrahymena thermophila and T. pigmentosa: evolutionary relationships in Tetrahymena MT family.嗜热四膜虫和色素四膜虫中四个新金属硫蛋白基因的克隆与测序：四膜虫金属硫蛋白家族的进化关系
Protist. 2003 Oct;154(3-4):431-42. doi: 10.1078/143446103322454167.
3
Metal interaction and regulation of Tetrahymena pigmentosa metallothionein genes.金属对四膜虫金属硫蛋白基因的相互作用与调控
Protist. 2002 Sep;153(3):283-91. doi: 10.1078/1434-4610-00105.
4
A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila.一个强大的可诱导-可抑制启动子极大地促进了嗜热四膜虫中基因敲除、条件表达以及同源和异源基因的过表达。
Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3734-9. doi: 10.1073/pnas.052016199. Epub 2002 Mar 12.
5
Functional genomics: the coming of age for Tetrahymena thermophila.功能基因组学：嗜热四膜虫的时代来临
Trends Genet. 2002 Jan;18(1):35-40. doi: 10.1016/s0168-9525(01)02560-4.
6
Identification, cloning and characterisation of a novel copper-metallothionein in tetrahymena pigmentosa. Sequencing of cDNA and expression.梨形四膜虫中一种新型铜金属硫蛋白的鉴定、克隆及特性分析。cDNA测序与表达
Protist. 2001 Sep;152(3):219-29. doi: 10.1078/1434-4610-00061.
7
Induction, regulation, degradation, and biological significance of mammalian metallothioneins.哺乳动物金属硫蛋白的诱导、调节、降解及生物学意义
Crit Rev Biochem Mol Biol. 2000;35(1):35-70. doi: 10.1080/10409230091169168.
8
Cadmium metallothionein gene of Tetrahymena pyriformis.
Gene. 1999 Jun 24;234(1):51-9. doi: 10.1016/s0378-1119(99)00181-x.
9
Surface display of a parasite antigen in the ciliate Tetrahymena thermophila.寄生虫抗原在嗜热四膜虫中的表面展示。
Nat Biotechnol. 1999 May;17(5):462-5. doi: 10.1038/8638.
10
Ciliates display promise for foreign gene expression.
Nat Biotechnol. 1999 May;17(5):424-5. doi: 10.1038/8590.

来自嗜热四膜虫的金属硫蛋白基因，带有一个铜诱导-可抑制启动子。

Metallothionein gene from Tetrahymena thermophila with a copper-inducible-repressible promoter.

作者信息

Boldrin Francesco, Santovito Gianfranco, Gaertig Jacek, Wloga Dorota, Cassidy-Hanley Donna, Clark Theodore G, Piccinni Ester

机构信息

Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35100 Padova, Italy.

出版信息

Eukaryot Cell. 2006 Feb;5(2):422-5. doi: 10.1128/EC.5.2.422-425.2006.

DOI:10.1128/EC.5.2.422-425.2006
PMID:16467482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1405887/
Abstract

We describe a novel metallothionein gene from Tetrahymena thermophila that has a strong copper-inducible promoter. This promoter can be turned on and off rapidly, making it a useful system for induction of ectopic gene expression in Tetrahymena and enhancing its applications in cell and molecular biology, as well as biotechnology.

摘要

我们描述了一种来自嗜热四膜虫的新型金属硫蛋白基因，它具有一个强铜诱导型启动子。该启动子能够快速开启和关闭，使其成为嗜热四膜虫中异位基因表达诱导的有用系统，并增强其在细胞生物学、分子生物学以及生物技术中的应用。