来自嗜热四膜虫的金属硫蛋白基因，带有一个铜诱导-可抑制启动子。
Metallothionein gene from Tetrahymena thermophila with a copper-inducible-repressible promoter.
作者信息
Boldrin Francesco, Santovito Gianfranco, Gaertig Jacek, Wloga Dorota, Cassidy-Hanley Donna, Clark Theodore G, Piccinni Ester
机构信息
Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35100 Padova, Italy.
出版信息
Eukaryot Cell. 2006 Feb;5(2):422-5. doi: 10.1128/EC.5.2.422-425.2006.
Abstract
We describe a novel metallothionein gene from Tetrahymena thermophila that has a strong copper-inducible promoter. This promoter can be turned on and off rapidly, making it a useful system for induction of ectopic gene expression in Tetrahymena and enhancing its applications in cell and molecular biology, as well as biotechnology.
摘要
我们描述了一种来自嗜热四膜虫的新型金属硫蛋白基因，它具有一个强铜诱导型启动子。该启动子能够快速开启和关闭，使其成为嗜热四膜虫中异位基因表达诱导的有用系统，并增强其在细胞生物学、分子生物学以及生物技术中的应用。
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