Lee So-Young, Kim Hyung-Jun, Park Sung-Jin, Yoon Hye-Jin, Yoon San-Hyun, Lee Kyung-Hoon, Lee Won-Don, Lim Jin-Ho
In Vitro Fertilization Laboratory, Maria Infertility Hospital, Dongdaemun-gu, Seoul, South Korea.
J Assist Reprod Genet. 2006 Feb;23(2):87-91. doi: 10.1007/s10815-005-9006-0. Epub 2006 Feb 9.
To verify a more effective dilution method that can be applied to human expanded blastocysts that are vitrified after artificial shrinkage.
Surplus expanded blastocysts that remained after embryo transfer (ET) in in vitro fertilization (IVF) cycles, were cryopreserved. The blastocysts were vitrified on EM grids following artificial shrinkage. After thawing the blastocysts, cryoprotectants were diluted using either a 6- or 2-step method. We examined the survival rate and clinical outcome of blastocysts of 151 patients in our ET program after thawing.
The survival rate of blastocysts that were thawed using a 2-step method (91.6%, 239/261) was comparable with that of the 6-step method (89%, 186/209). The clinical pregnancy rate (45.9%, 39/85) and implantation rate (24.1%, 53/220) were slightly higher in the 2-step method than in the 6-step method (40.9%, 27/66; 19.4%, 33/170).
Our data indicate that the 2-step dilution method could be a simpler and more effective protocol for human expanded blastocysts that are vitrified using EM-grid following artificial shrinkage.
验证一种更有效的稀释方法，该方法可应用于人工收缩后玻璃化的人类扩张期囊胚。
将体外受精（IVF）周期中胚胎移植（ET）后剩余的扩张期囊胚进行冷冻保存。在人工收缩后，将囊胚在电子显微镜网格上进行玻璃化。解冻囊胚后，使用6步或2步方法稀释冷冻保护剂。我们检查了151例患者在ET程序中解冻后囊胚的存活率和临床结局。
使用2步方法解冻的囊胚存活率（91.6%，239/261）与6步方法（89%，186/209）相当。2步方法的临床妊娠率（45.9%，39/85）和着床率（24.1%，53/220）略高于6步方法（40.9%，27/66；19.4%，33/170）。
我们的数据表明，对于人工收缩后使用电子显微镜网格玻璃化的人类扩张期囊胚，2步稀释方法可能是一种更简单、更有效的方案。
