Vanderzwalmen P, Bertin G, Debauche Ch, Standaert V, Bollen N, van Roosendaal E, Vandervorst M, Schoysman R, Zech N
Schoysman Infertility Management Foundation, AZ Jan Portaels, Gendarmeriestraat 65, 1800 Vilvoorde, Belgium.
Hum Reprod. 2003 Jul;18(7):1504-11. doi: 10.1093/humrep/deg298.
The present study was undertaken to examine the usefulness of both vitrification and assisted hatching (AH) on blastocysts that originate from embryos showing different qualities during their cleavage stage.
A total of 281 blastocysts were vitrified (93 vitrification-warming cycles) in a mixture of ethylene glycol-dimethylsulphoxide-Ficoll and sucrose using the Hemi-Straw (HS) carrier system. After warming, AH using the partial dissection technique was performed in 36 cycles.
After warming and culture for 24 h, a total of 168 blastocysts (60%) was suitable for embryo transfers and a total of 25 ongoing pregnancies (27%) was obtained. Forty-nine transfers of 96 no-AH blastocysts and 36 transfers of 72 AH blastocysts resulted in an implantation rate of 13 and 22% respectively (P < 0.05). The percentage of transfers with at least one hatching blastocyst was significantly higher after application of AH (69 versus 33%) (P < 0.001). In all, 73 and 38% of blastocysts showing respectively optimal and non-optimal embryo development during the early stage were available for transfer (P < 0.001). Consequently, implantation rates of 19 and 6% were obtained after transfers of blastocysts showing respectively optimal and poor embryo development.
Artificial opening of the zona pellucida after warming of vitrified blastocysts significantly improved the rate of transfers with hatched blastocysts and the implantation and pregnancy rates. The percentage of blastocysts that survived the HS vitrification procedure and were available for embryo transfer is related to their previous developmental quality.
本研究旨在探讨玻璃化冷冻和辅助孵化（AH）对来自卵裂期表现出不同质量胚胎的囊胚的有效性。
使用半麦管（HS）载体系统，将总共281个囊胚在乙二醇 - 二甲基亚砜 - 聚蔗糖和蔗糖的混合物中进行玻璃化冷冻（93次玻璃化 - 复温循环）。复温后，在36个循环中使用部分切割技术进行辅助孵化。
复温并培养24小时后，总共168个囊胚（60%）适合胚胎移植，共获得25例持续妊娠（27%）。96个未进行辅助孵化的囊胚进行了49次移植，72个进行辅助孵化的囊胚进行了36次移植，着床率分别为13%和22%（P < 0.05）。应用辅助孵化后，至少有一个孵化囊胚的移植百分比显著更高（69%对33%）（P < 0.001）。总体而言，早期分别显示最佳和非最佳胚胎发育的囊胚中，73%和38%可用于移植（P < 0.001）。因此，分别移植显示最佳和较差胚胎发育的囊胚后，着床率分别为19%和6%。
玻璃化囊胚复温后人工打开透明带显著提高了孵化囊胚的移植率、着床率和妊娠率。在HS玻璃化冷冻程序中存活并可用于胚胎移植的囊胚百分比与其先前的发育质量有关。
