Hintzen R Q, de Jong R, Hack C E, Chamuleau M, de Vries E F, ten Berge I J, Borst J, van Lier R A
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
J Immunol. 1991 Jul 1;147(1):29-35.
The human T cell Ag CD27 belongs to a recently defined family of cell surface receptors, including the nerve growth factor receptor, two distinct tumor necrosis factor receptors, and the B cell specific molecule CD40. On resting T cells, CD27 is a transmembrane homodimer with subunits of 50 to 55 kDa (p55). T cell activation via the TCR/CD3 complex causes a strong enhancement of p55 expression. Concomitantly, an alternative form of the CD27 molecule with a molecular mass of 28 to 32 kDa (p32) appears at the cell surface. With the use of ELISA, we here show that a soluble form of CD27 (sCD27) can be detected in the supernatant of T cells activated with anti-CD3 or combinations of anti-CD2 mAb. Moreover, sCD27 is found in both serum and urine from healthy donors. sCD27, purified from either culture supernatant or urine, has a molecular mass of 28 to 32 kDa and is, according to peptide mapping, structurally homologous to the p55 membrane form of CD27. Quantification of sCD27 levels may be used as a marker for T lymphocyte activation in vivo.
人类T细胞抗原CD27属于最近定义的细胞表面受体家族，包括神经生长因子受体、两种不同的肿瘤坏死因子受体以及B细胞特异性分子CD40。在静息T细胞上，CD27是一种跨膜同型二聚体，亚基分子量为50至55 kDa（p55）。通过TCR/CD3复合物激活T细胞会导致p55表达强烈增强。与此同时，一种分子量为28至32 kDa（p32）的CD27分子的替代形式出现在细胞表面。通过酶联免疫吸附测定法（ELISA），我们在此表明，在用抗CD3或抗CD2单克隆抗体组合激活的T细胞上清液中可检测到可溶性CD27（sCD27）。此外，在健康供体的血清和尿液中均发现了sCD27。从培养上清液或尿液中纯化的sCD27分子量为28至32 kDa，根据肽图谱分析，其结构与CD27的p55膜形式同源。sCD27水平的定量测定可作为体内T淋巴细胞激活的标志物。
