CHU Clermont-Ferrand, Polyclinique-Hôtel-Dieu, Gynécologie Obstétrique et Médecine de la Reproduction, Boulevard Léon Malfreyt, 63058 Clermont-Ferrand, France.
Hum Reprod. 2010 Mar;25(3):742-50. doi: 10.1093/humrep/dep435. Epub 2009 Dec 10.
The objective of the present study was to expand our understanding of the role of matrix metalloproteinase-7 (MMP-7) in the pathophysiology of endometriosis.
Expression levels of MMP-7 mRNA and protein in the eutopic endometrium and ectopic endometrium of patients with different forms of endometriosis were measured with immunohistochemistry and real-time RT-PCR. Endometrial tissues from patients with uterine myomas and those with macroscopically normal pelvic cavities were included as comparison groups. The real-time RT-PCR utilized endometrial cells isolated by laser capture microdissection. MMP-7 immunostained cells were quantified using a computerized image analysis system.
MMP-7 expression levels were significantly higher in the endometrial epithelial cells from patients with deep infiltrating endometriosis compared with those isolated from the endometria of patients with only superficial peritoneal endometriosis, uterine myomas or normal endometrium, in the proliferative, late secretory and menstrual phases. MMP-7 protein expression was detected in the ectopic endometrial epithelial cells of 13 samples of deep infiltrating endometriosis (24.5%), 11 samples of ovarian endometriosis (28.6%), 23 samples of black peritoneal lesions (76.7%) and 24 samples of red peritoneal lesions (100%). MMP-7 protein expression in epithelial cells was significantly higher in red peritoneal lesions compared with that of deep infiltrating endometriosis, ovarian endometriosis and black peritoneal lesions, in all phases of the menstrual cycle.
These findings suggest that MMP-7 expression levels vary significantly among the different forms of endometriosis.
本研究旨在深入了解基质金属蛋白酶-7（MMP-7）在子宫内膜异位症病理生理学中的作用。
采用免疫组化和实时 RT-PCR 检测不同类型子宫内膜异位症患者在位和异位内膜中 MMP-7 mRNA 和蛋白的表达水平。将子宫肌瘤患者和盆腔大体正常患者的子宫内膜作为对照组。实时 RT-PCR 利用激光捕获显微切割分离的子宫内膜细胞进行检测。采用计算机图像分析系统对 MMP-7 免疫染色细胞进行定量。
与仅患有浅表腹膜子宫内膜异位症、子宫肌瘤或正常子宫内膜的患者相比，深度浸润性子宫内膜异位症患者的子宫内膜上皮细胞中 MMP-7 表达水平在增殖期、晚期分泌期和月经期均显著升高。在 13 例深度浸润性子宫内膜异位症（24.5%）、11 例卵巢子宫内膜异位症（28.6%）、23 例黑色腹膜病变（76.7%）和 24 例红色腹膜病变（100%）的异位子宫内膜上皮细胞中均检测到 MMP-7 蛋白表达。在整个月经周期中，红色腹膜病变的上皮细胞中 MMP-7 蛋白表达均显著高于深度浸润性子宫内膜异位症、卵巢子宫内膜异位症和黑色腹膜病变。
这些发现表明 MMP-7 表达水平在不同类型的子宫内膜异位症中存在显著差异。
