Chen Ssu-Ching, Chen Ching-Hsein, Chern Chi-Liang, Hsu Li-Sung, Huang Ya-Chun, Chung King-Thom, Chye Soi-Moi
Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, Taiwan.
Toxicol In Vitro. 2006 Sep;20(6):801-7. doi: 10.1016/j.tiv.2005.12.003. Epub 2006 Feb 15.
The mechanism of toxicity p-phenylenediamine (p-PD), a component of human permanent hair dye and a suspected carcinogen, on the growth of Mardin-Darby canine kidney cells (MDCK) was investigated. With the analysis of flow cytometry, a dose-dependent accumulation of the sub-G1 peak and the G0/G1-phase arrested in cell cycle, and time-dependent induction of apoptosis after staining with Annexin V-Fluorescein and propidium iodide were observed. After the treatment of cells with p-PD, dose dependent DNA fragmentation shown by gel electrophoresis, the reduction of membrane potential (DeltaPsim) by mitochondria membrane depolarization and the increase of the expression of p53 protein in cells, suggested that the effect of p-PD on overall viability and cell numbers is mediated by an increase in apoptosis.
对作为人类永久性染发剂成分且疑似致癌物的对苯二胺（p-PD）对马尔堡-达尔比犬肾细胞（MDCK）生长的毒性作用机制进行了研究。通过流式细胞术分析，观察到亚G1峰呈剂量依赖性积累，细胞周期停滞于G0/G1期，在用膜联蛋白V-荧光素和碘化丙啶染色后，细胞凋亡呈时间依赖性诱导。用p-PD处理细胞后，凝胶电泳显示剂量依赖性DNA片段化、线粒体膜去极化导致膜电位（ΔΨm）降低以及细胞中p53蛋白表达增加，这表明p-PD对总体活力和细胞数量的影响是由细胞凋亡增加介导的。
